To verify the composition and the chemical variation of its essential oil along 24 hours, leaves of patchouli of medicinal plant collection of the Department of Plant Production/Horticulture of UNESP-Botucatu-SP, were collected every three hours during one day (of the 06:00 to 03:00 h) between February, 25 and 26, 2001. The leaves had its oil extracted for hydro-distillation in Clevenger apparatus during three hours with two repetitions. The chemical composition analysis of the extracted essential oils was carried out in gas chromatography/mass spectroscopy. The phytochemical profile of the essential oils did not present significant difference in function of the harvest. The five most abundant were: patchouli alcohol (62.88 to 70.74 %), αbulnesene (55.12 to 6.84 %), α-guaiene (2.87 to 4.25 %), γ-patchoulene (2.66 to 3.52 %) and another sesquiterpene (5.16 to 6.56 %) not identified, with retention time near to the major compounds.
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