One of the important Integrated Pest Management Strategies (IPM) against economic pests in Egypt is the use of biological control methods.The use of entomopathogenic bacteria is one of these methods. The authors achieved four isolates of bacteria from the naturally infected P. gossypiella in cotton fields at Assuit and Kafr El-Sheikh Governorates, Egypt. Isolates were characterized based on morphological properties. Morphological analyses were carried out based on Bergey's Manual of Systematic Bacteriology. The identification of the isolated bacteria was confirmed by using Biolog-System as, Staphyloccus sciuri, Micrococcus luteus, Brevibacterium lines & Brevibacterium casei. In addition, their larvicidal effect was evaluated. The results revealed that, S. sciuri and M. luteus were the most effective against 1 st instar larvae of PBW. Furthermore, their latent effect caused the lowest pupation % resulted from treated PBW larvae. S. sciuri was the most promising bacterial isolate to be developed as a biological control agent against P.gossypiella. The antagonists of bacterial strain against the mycelial growth of the pathogenic fungi (Rhizoctonia solani, Fusarium solani and Macrophomia phaseolina), was experimently tested to evaluate growth reduction of all pathogenic fungi.

Field studies were conducted at Dakahlia Governorate, Egypt in 2004 and 2005 cotton seasons to evaluate the releases of Trichogramma wasps for pink bollworm, Pectinophora gossypiella (Saund.) and spiny bollworms Earias insulana (Boisd.) management in cotton. In the two seasons, six releases of Trichogramma evanescens Westwood 10-15 days intervals were made into 999 and 600 feddan, respectively. While, the recommended bollworms insecticide control program was applied at the control units. This recommended program suggested to use insecticide applications (insect growth regulators, organophosphoric and pyrethroid compounds) when infestation met or exceed the economic level (3%). The overall reduction of infestation with the two pests in Trichogramma units averaged 23.73 and 40%, since infestation averaged 1.35 & 1.44.0 in these units and 1.77 & 2.4% in control units at the two seasons, respectively. Meanwhile, T. evanescens succeeded to maintain boll infestation below the economic level in 78 and 84% of the dissected boll samples in the two seasons, respectively. The corresponding figures by insecticides were 67 and 56% in the two seasons, respectively.

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