Over the last 25 years, numerous studies have investigated the impact of insect supplementation on insect nutrient content. In light of recent nutrition related challenges with regards to zoo amphibians fed an insect based diet, this review attempts to comprehensively compile both anecdotal and published data in the context of practical application on this topic. Insects, primarily crickets, used for amphibian diets historically demonstrate low concentrations of key nutrients including calcium and vitamin A. Commonly used practices for supplementation involving powder dusting or gut loading have been shown to improve delivery of calcium and vitamin A, though often not reaching desired nutrient concentrations. The large variety of factors influencing insect nutrient content are difficult to control, making study design, and results often inconsistent. Formulation and availability of more effective gut loading diets, combined with a standardized protocol for insect husbandry and dietary management may be the most effective way to supplement insects for use in amphibian feeding programs. Ideally, the nutritional improvement of feeder insects would begin at the breeder level; however, until this becomes a viable choice, we confirm that supplementation of crickets through both gut-loading and dusting appear necessary to support the nutritional health of amphibians and other insectivores in managed collections.

Background: Theraphosidae is a large family of tarantula spiders commonly kept as pets or display animals by zoological institutions. Interest and demand for arachnid medicine have grown over the last decade. While hemolymph analysis could be a fundamental tool for arachnid health assessment, RIs are generally lacking for arachnid species.Objectives: The objectives of this study were to describe the appearance of hemocytes and establish RIs for total and differential hemocyte counts as well as hemolymph glucose and electrolyte concentrations in the Cameroon red tarantula (CRT) (Hysterocrates gigas).Methods: Ninety-three CRTs were anesthetized using isoflurane or sevoflurane, and hemolymph was collected from the heart. Hemocyte counts were performed using a hemocytometer and trypan blue stain, and differential cell counts were estimated using light microscopy and a Wright stain. Hemocytes were also characterized with transmission electron microscopy (TEM). Hemolymph glucose and electrolyte concentrations were obtained using a blood gas analyzer. The American Society for Veterinary Clinical Pathology (ASVCP) guidelines were used to calculate RIs. Results:The most abundant hemocytes were plasmatocytes, followed by granulocytes, cyanocytes, and prohemocytes. Hemocytes were characterized by TEM except for cyanocytes. All RIs had a broad range, likely due to a combination of population heterogeneity, high biologic variability of the species, and the propensity of samples to clot. Conclusions:The current study is the first to establish RIs for hemolymph cytologic parameters with a sample size of over 20 individuals in any theraphosid species. RIs for CRT hemolymph biochemical analytes are also reported. K E Y W O R D S arachnids, electrolytes, hemocyte, hemolymph, reference intervals, tarantulas 1 | INTRODUC TI ON Theraphosid tarantulas are commonly kept as pets by hobbyists and as display animals by zoological institutions. The Cameroon red tarantula (CRT) (Hysterocrates gigas), also known as the giant baboon spider, is a burrowing species that originates from Cameroon in Western Africa. Compared with females, adult males have a smaller opisthosoma, and the ends of their pedipalp tarsi

A die-off occurred in a captive colony of blue-winged grasshoppers (Tropidacris collaris) at the Toronto Zoo. One fourth of the colony died within a year due to infection with worms initially mistaken for nematomorphs but later identified as nematodes belonging to the Mermithidae, genus Mermis. Mortality persisted and the grasshopper population dwindled over the following years. Mermithid larvae developed in the hemocoel of the insects until they eventually emerged from a hollowed-out exoskeleton. Circumstantial evidence suggests that the parasites were introduced with raspberry browse that was grown on site and contaminated with mermithid eggs.