The
specificity of fructooligosaccharides as prebiotics depends
on their size and structure, which in turn depend on their origin
or the synthesis procedure. In this work we describe the application
of an inulosucrase (IslA) from Leuconostoc citreum CW28 to produce high molecular weight inulin from sucrose alongside
a commercial endoinulinase (Novozym 960) produced by Aspergillus
niger for a simultaneous or sequential reaction to synthesize
fructooligosaccharides (FOS). The simultaneous reaction resulted in
a higher substrate conversion and a wide diversity of FOS when compared
to the sequential reaction. A shotgun MS analysis of the commercial
endoinulinase preparation surprisingly revealed an additional enzymatic
activity: a fructosyltransferase, responsible for the synthesis of
FOS from sucrose. Consequentially, the range of FOS obtained in reactions
combining inulosucrase from Ln. citreum with the
fructosyltransferase and endoinulinase from A. niger with sucrose as substrate may be extended and regulated.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.