Soft conductors show great promise in multifunctional sensor applications. However, both electronically and ionically conductive materials are often vulnerable during large deformation or at low temperatures, leading to reduced sensitivity,...
Numerous analytical techniques have been undertaken for the detection of protein biomarkers because of their extensive and significant applications in clinical diagnosis, whereas there are few strategies to develop dual-readout immunosensors to achieve more accurate results. To the best of our knowledge, inspired by smart drug delivery system (DDS), a novel pH-responsive modified enzyme-linked immunosorbent assay (ELISA) was innovatively developed for the first time, realizing dual-modal colorimetric and fluorescent detection of cardiac troponin I (cTnI). Curcumin (CUR) was elaborately selected as a reporter molecule, which played the same role of drugs in DDS based on the following considerations: (1) CUR can be used as a kind of pH indicator by the inherited allochroic effect induced by basic pH value; (2) the fluorescence of CUR can be quenched by certain nanocarriers as the acceptor because of the occurrence of fluorescence resonance energy transfer (FRET), while recovered by the stimuli of basic pH value, which can produce "signal-on" fluorescence detection. Three-dimensional MoS nanoflowers (3D-MoS NFs) were employed in immobilizing CUR to constitute a nanoprobe for the determination of cTnI by virtue of good biocompatibility, high absorption capacity, and fluorescence quench efficiency toward CUR. The proposed DDS-inspired ELISA offered dual-modal colorimetric and fluorescent detection of cTnI, thereby meeting the reliable and precise analysis requirements. We believe that the developed dual-readout ELISA will create a new avenue and bring innovative inspirations for biological detections.
An enzyme-free titer plate-based colorimetric assay utilizing functionalized mesoporous silica nanoparticles (MSNs) entrapping pH-indicator molecules has been developed. Pores in the silica nanoparticles were functionalized with phenyltrimethyloxysilane so that pH indicator molecules (thymolphthalein or TP in the present case) can be tightly entrapped through π-π conjugation. To detect prostate specific antigen (PSA), the TP-containing MSNs were coated with polyethylenimine (PEI), which favors the attachment of the negatively charged secondary anti-PSA antibody. The entrapped thymolphthalein molecules can be readily released from the pores with a simple addition of alkaline solution. The resultant bifunctional MSNs were used for signal-amplified detection of PSA captured by the primary antibody preimmobilized in the wells of a plate. Our method possesses a wide dynamic range (0.5 to 8000 pg/mL) wherein the adsorption of the bifunctional MSNs obeys a modified Langmuir isotherm. A detection limit (LOD) down to as low as 0.36 pg/mL can be attained. Owing to the size uniformity of the MSNs and the obviation of enzyme molecules employed in the enzyme-linked immunosorbent assay (ELISA), excellent reproducibility (RSD = 1.12%) was achieved. The selective detection of PSA in human serum samples demonstrates the amenability of our method to detect important biomarkers in complex biological samples, whereas the performance of the assay in a titer plate ensures high throughput and obviates the use of expensive instruments. Both of these features are prerequisites for clinical settings wherein a great number of samples need to be analyzed in a timely fashion.
Functionalized
materials with reversible color switching are highly
attractive in many application fields, especially as rewritable media
for information storage. It is critical yet challenging to develop
a cost-effective strategy for the fabrication of stimulus-responsive
chromogenic systems. Herein, we present a versatile dip-coating approach
to fabricate robust smart textile with acid/base-driven chromotropic
capability. Owing to the introduction of novel maleimide-based copolymers
bearing azobenzene derivative moieties, smart textiles possess rapid
color switching between yellow and orange-red, which is triggered
by acid–base stimulations with the resulting reversible protonation/deprotonation
of maleimide moieties. As a proof of concept of the application of
the smart textile for high-performance rewritable media, various rewritable
elaborate patterns can be fast trifluoroacetic acid-printed/triethylamine-erased
(within 20 s) with excellent cycling stability and long legible duration
(>30 days). Meanwhile, the smart textile can be employed as a visual
sensor for the detection of hydrogen fluoride gas leakage. It is highlighted
that the as-prepared robust smart textiles with superhydrophobic surfaces
have excellent antifouling properties and chemical/mechanical stabilities,
which can tolerate harsh environmental conditions and repetitive mechanical
deformation. The robust smart textiles with simple low-cost large-scale
production may find more advanced potential applications besides information
storage and sensors demonstrated.
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