FeO/Ag core/shell nanoparticles functionalized with the free amino (NH) functional groups (FeO/Ag-NH) were conjugated with fluorescent electron coupled dye (ECD)-antiCD34 antibody using the 1-ethyl-3-(3'-dimethyl-aminopropyl) carbodiimide (EDC) catalyst (ECD - Electron Coupled Dye or R Phycoerythrin-Texas Red is a fluorescent organic dye attached to the antibody). The characteristic fluorescence of ECD in the antibody was investigated and was used as a good indicator for estimating the percentage of the antibodies that were successfully conjugated with the nanoparticles. The conjugation efficiency was found to increase depending on the V:V ratio, where V and V are the volumes of the nanoparticle solution (concentration of 50 ppm) and the as-purchased antibody solution, respectively. The conjugation efficiency rapidly increased from approximately 18% to approximately 70% when V:V was increased from 2:1 to 100:1, and it gradually reached the saturated state at an efficiency of 95%, as the V:V was equal to 300:1. The bioactivity of the abovementioned conjugation product (denoted by FeO/Ag-antiCD34) was evaluated in an experiment for the collection of stem cells from bone marrow samples.
a b s t r a c tZnS:MneFe 3 O 4 bifunctional nanoparticles were synthesized by inverse microemulsion method for biomedicine applications. The bifunctional nanoparticles were combined from prepared ZnS:Mn and Fe 3 O 4 nanoparticles in a SiO 2 cover matrix. Results show that bifunctional nanoparticles, apart from exhibiting magnetism, have photoluminescence properties, which support the applications targeting biomedicine fluorescent diagnostics as well as magnetic cell sorting or drug delivery.
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AbstractThe surface-enhanced Raman (SER) signal of 4-aminothiolphenol (4-ATP) based on the surface of gold nanoparticles colloids with size distributed from 2 to 5 nm were used as labeling agent to detect basal cell carcinoma (BCC) of skin. The enhanced Raman band at 1075 cm -1 corresponding to C-S stretching vibration in 4-ATP was observed during attachement to the surface of gold nanoparticles. The frequency and intensity of this band did not change when the colloids were conjugated with BerEP4 antibody which specifically binds to BCC. We show the feasibility of imaging BCC by SERS scanning the 1075 cm -1 band to detect the distribution of 4-ATP-attached gold nanoparticles attached to skin tissue ex-vivo.
This work focuses on the synthesis, functionalization, and application of gold and silver nanoparticles, magnetic nanoparticles Fe 3 O 4 , combination of 4-ATP-coated silver nanoparticles and Fe 3 O 4 nanoparticles. The synthesis methods such as chemical reduction, seeding, coprecipitation,and inverse microemulsion will be outlined. Silica-and amino-coated nanoparticles are suitable for several applications in biomedicine and the environment. The applications of the prepared nanoparticles for early detection of breast cancer cells, basal cell carcinoma, antibacterial test, arsenic removal from water, Herpes DNA separation, CD4 + cell separation and isolation of DNA of Hepatitis virus type B (HBV) and Epstein-Barr virus (EBV) are discussed. Finally, some promising perspectives will be pointed out.
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