Bioremediation is a biological process to remove or neutralize environmental pollutants. This study was carried out to investing at the efficacy of arsenic resistant endophytic bacteria isolated from Pteris vittata, Pityrogramma calomelanos, Blenchum orientale, and Nephrolepis exaltata, which grow in a highly arsenic (As) contamination mining site in Vietnam. Their segmented roots, stems, and leaves were homogenized separately and inoculated on LB agar plates containing 5mM As(III) and As(V). A total of 31 arsenic resistant endophytic strains were selected, in which strain R2.5.2 isolated from the root of P. calomelanos had the highest arsenic resistant capability. Strain R2.5.2 tolerated up to 320 mM and 160 mM of arsenate and arsenite, respectively. The strain developed well on a media of 0.1 5% NaCl, at 20-40ºC and pH 5 9, and actively utilized most of the sugar sources. It had a high IAA biosynthesis capacity with an average concentration of 19.14 mg/L, tolerated to 0.5-16 mM concentration of Ag+, Hg2+, Co2+, Ni2+, Cu2+, Cr4+, and reduced As(V). Based on 16s rDNA, R2.5.2 was identified as Priestia megaterium. The ars C gene coding for arsenate reductase catalyzing reduction of As(V) was successfully amplified in P. megaterium R2.5.2. The selected strain may have potential use for bioremediation practice.
The excess use of pesticides in the agricultural sector has caused environmental pollution and affected the complete ecosystem. Among the various commonly used pesticides, chlorpyrifos (CPF) is widely used against multiple agrarian pests due to its effectiveness and higher insecticidal activities. However, along with its beneficial usage, CPF has various residual effects on the environment, causing multiple negative impacts on aquatic organisms and human health. Consequently, methods for eliminating CPF in the background are essential. Among the currently available approaches to CPF remediation, biological methods using microorganisms are eco-friendly and cost-effective. Therefore, this study was conducted to isolate and characterize chlorpyrifos-degrading bacteria from the tea-growing soil of Vietnam. For this, soil samples were collected from the 20 tea-growing areas of Vietnam. From the collected samples, three bacterial strains viz., Methylobacterium populi CNN2, Ensifer adhaerens VNN3, and Acinetobacter pittii CNN4 have been isolated by using streak plate method and identified based on 16S rRNA gene analysis. The study results showed that under laboratory conditions, E. adhaerens VNN3 had the highest CPF degradation ability and was followed by the strain M. populi CNN2. In liquid medium, CPF concentration (100 mg/L) was reduced by 95.2% and 81.4% by E.adhaerens VNN3 and M. populi CNN2, respectively, after 72 h. Further, under in-vitro conditions, the concentration of CPF was reduced from 500 mg/kg to 112 ± 1.73 (77.6%) and 197 ± 2.08 mg/kg (60.6%) by E. adhaerens VNN3 and M. populi CNN2, respectively. Based on the obtained results, it can be concluded that E. adhaerens VNN3 and M. populi CNN2 can be used for CPF-contaminated agricultural soil remediation.
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