An enzyme-linked immunosorbent assay (ELISA) was developed to estimate the quantity of Flavobacterium branchiophilum in crude gill extracts from rainbow trout Oncorhynchus mykiss following bath exposure to the bacterium. The assay utilized the avidin-biotin system and polyclonal antiserum raised against the LAB 4a strain of F: branchiophilum. The detection threshold was ca 1 X 10"acteria rnl-', and during routine use the mean intra-assay and inter-assay variations were 6.7 % and 8.l'%, respectively The ELISA absorbance (405 nm) was proportional to the amounl of F branrh~o-phjlum present (within a range of antigen concentration of 0 to 80 000 cells ml-') whether whole bacterial cell preparations, g111 preparations splked with bacterial cells or extracts of infected gills were tested. In a comparison of whole cell preparations derived from the type strain of F branchiophilum (Amencan Type Culture Collection 35035), the LAB 4a strain and other common gill isolates (4 Flavobacten'um sp., a Flexlbactersp. and Aeromonas hydrophila), the assay proved specific for F branchiophiluni antigen. Adaption for field-collected samples is feasible, but will require further examination of the antigenic specificity, and re-optimization of the tissue sample concentration if gills from other species are to be tested. The ELISA is an achievable means of estimating the quantity of F: branchiophilum on the gills of large numbers of fish, and represents an important tool for bacterial gill disease research.

CAR bacillus infection should be considered as a differential diagnosis for pneumonia in rats in New Zealand.

Watery mouth was diagnosed as the cause of illness and subsequent death in a 3-day-old Romney lamb which had not received early colostrum. The history, clinical signs and post-mortem findings were typical of the disease as seen in other countries, including Great Britain. The diagnosis was based on the clinical signs and was supported by an absence of post-mortem findings of other specific neonatal diseases. The potential importance of watery mouth under conditions of intensive husbandry in New Zealand is discussed.