To facilitate the routine identification of salmonella and detailed studies of their lipopolysaccharides, we raised murine monoclonal antibodies against these organisms. We raised an immunoglobulin GI antibody, M02, which is specific for factor 02. By immunoblotting following sodium dodecyl sulfate-polyacrylamide gel electrophoresis, M02 was shown to bind only the lipopolysaccharide of Salmonella paratyphi A, giving a ladderlike reaction pattern with regularly spaced reactive bands. M02 did not react against lipopolysaccharides of other Salmonella serogroups, including those of serogroups B, C, D, E, and L. Since the lipopolysaccharides of Salmonella serogroups A, B, D, and E are similar except for the presence of paratose in serogroup A organisms, this dideoxyhexose is therefore believed to be the immunodominant epitope for M02. Consistent with the latter contention was the finding that periodate oxidation of the S. paratyphi A lipopolysaccharide did not destroy its antigenicity for M02. In a slide agglutination test, M02 was found to react specifically against al 12 clinical isolates of S. paratyphi A but not against 98 isolates of other salmonella or 74 isolates of other bacteria and Candida albicans.
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