ABSTRACT. ULTRASTRUCTURAL STUDY OF TH E STOMACH OF TRICHOGENES LONGIPINNIS (BRITSKI & ORTEGA) (PISCES , SILURIFORMES, TRICHOMICTERlDAE). The obselVations made abo ut the stomach ofTrichogenes longipinnis (Britski & Ortega, 1983) showed a rounded organ which has in its dorsal side an accessory structure -a cul de sac -that comunicates with it. The distal part ofthis dorsal accessorypresents characteristic of an organ that exerci se respiratory functions: reduction in the thickness of the mucosa and arrangement of an intrincate continuous capilar net in dose contact with lhe superficial cells of the epilhelium. ln this portion Ihere are no glands in the lamina propria. The epithelium shows a superficial squamous cels; they are very slim, and sometimes, with the capillary endothelium form a single barrier between the organ lumen and lhe blood. Polyhedric cells are also present in this epithelium; they are located under the squamous cells, sheltered betwcen capillaries and basal cells.
ABSfRACf. ULTRASfRUCfURAL sruDY OF THE OESOPHAGOUS O F TRICHOGENES LONGIPINNIS (BRITSKI & ORTEGA) (PISCES, SILURIFORMES, TRICHOMICTERIDAE). Trichogenes longipinnls (Britski & Ortega, 1983) is a leather fish restricted to the rivers on the shore in the southeast of Brazil. ln this work, the oesophagous structure is being showed. The oesophagous appears itself short, dorsaliy inciinated toward pericardium and ventraliy covered by iiver lobe. By electron microscopy we can observe a mucosa layer formed by stratified squamous epithelium and lamina propria with slralum compactum. The epithelium is composed by three types of celis: superficial squamous celis; mucous celis, similar to the globet celis and polyhedric celis. The stratification of this epithelium provides a complete basal layer of proliferation polyhedric cells. The celis in the intermediate region of the epithelium are also polyhedric. These celis are juS! bellow the superficial squamous cells and packed among mucous celis.
A revie w o f th e literatur e o n myopathie s di d no t disclos e sufficien t dat ato characteriz e th e cas e her e presente d amon g th e classicall y describe d myopathies. Thus , th e author s carrie d ou t a clinical , a n histochemica l an d ultrastructural analisy s i n orde r t o contribut e t o th e characterizatio n o f thi s peculiar muscl e pathology . CASE REPORTThe patient, a three-year-old boy, was brought to our Muscle Disease Clinic in 1975, with a clinical history of difficulty for climbing stairs ever since he had learned to walk.He was said to have been in good health before this, even though he was only able to sit up without some kind of support at the age of 10 months and only started to walk at the age of 20 months.The clinical examination was normal, except for the presence of dome-shaped elevated palate; the neurological examination was normal, except for the presence of a slightly myopathic gait and the impossibility of climbing stairs without the help of his hands; myopathic rise; hypotony of lower limbs and a slight muscle hypotrophy of limb girdle and the femoral quadriceps muscles.EMG recording of femoral quadriceps, done bilaterally, revealed potentials of decreased amplitude and duration, as well as a decrease in the interference potenciais. The laboratory investigation showed a normal CPK and the urine test was normal as far as innate metabolic errors are concerned.A muscle biopsy from the quadriceps was performed. Part of this material was fixed in 10% neutral formalin and embedded in parafin.For light microscope observations, 7 um sections were stained with Hematoxilin and Eosin (H and E). For histochemistry, the following histochemical methods were used: periodic-acid-Schiff (PAS); PAS after alpha-amylase digestion for 30 minutes at 37©C; colloidal iron (Hale's method modified by Müller) and Alcian Blue (AB) at pH 2.5 (Pearse, 1968).For electron microscopy, fragments from the biopsy material were fixed in 2% glutaraldehyde in phosphate buffer, pH 7.2, and post-fixed in 1% osmium tetroxide and 0.5% uranyl acetate, both solutions at 380 mOsm (De Harven, 1967). Embedding was carried out in araldite resin (Luft, 1971). For light microscope observations, thick sections of 500 nm width, stained with toluidine blue were used.
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