No abstract

Denmark has an extraordinarily large and well-preserved collection of archaeological skin garments found in peat bogs, dated to approximately 920 BC – AD 775. These objects provide not only the possibility to study prehistoric skin costume and technologies, but also to investigate the animal species used for the production of skin garments. Until recently, species identification of archaeological skin was primarily performed by light and scanning electron microscopy or the analysis of ancient DNA. However, the efficacy of these methods can be limited due to the harsh, mostly acidic environment of peat bogs leading to morphological and molecular degradation within the samples. We compared species assignment results of twelve archaeological skin samples from Danish bogs using Mass Spectrometry (MS)-based peptide sequencing, against results obtained using light and scanning electron microscopy. While it was difficult to obtain reliable results using microscopy, MS enabled the identification of several species-diagnostic peptides, mostly from collagen and keratins, allowing confident species discrimination even among taxonomically close organisms, such as sheep and goat. Unlike previous MS-based methods, mostly relying on peptide fingerprinting, the shotgun sequencing approach we describe aims to identify the complete extracted ancient proteome, without preselected specific targets. As an example, we report the identification, in one of the samples, of two peptides uniquely assigned to bovine foetal haemoglobin, indicating the production of skin from a calf slaughtered within the first months of its life. We conclude that MS-based peptide sequencing is a reliable method for species identification of samples from bogs. The mass spectrometry proteomics data were deposited in the ProteomeXchange Consortium with the dataset identifier PXD001029.

No abstract

In recent years, extensive archaeological studies have provided us with new knowledge on wool and woollen textile production in continental Europe during the Bronze Age. Concentrations of large numbers of textile tools, and of zooarchaeological evidence suggesting intense sheepherding, hint at specialized centres of wool production during the Bronze Age. The aim of this paper is to discuss whether engagement with this economic activity was facilitated by the introduction of new foreign sheep types, possibly from the Eastern Mediterranean, where well-established wool economies existed, or by using local sheep, or a mixture of local and non-local types. A small-scale genetic pilot study, presented in this paper, primarily aimed at testing the DNA preservation, and thus the genomic potential in Bronze Age sheep remains provides evidence of both mitochondrial haplogroups A and B among Bronze Age sheep in Hungary. This result could hint at sheep herds with mixed origin but further in-depth studies are necessary to address this. We aim to promote scholarly interest in the issue and propose new directions for research on this topic.