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The staining time for mammalian skeletal muscle fixed in neutral phosphatebuffered formalin was shortened from 12-24 hr to 10-30 min. The permanganate-oxalate sequence was omitted although oxidation by periodic add or with iodine was found to be necep sary. The material was embedded in paraffin and cut 6 p or less. Depara5ized sections were treated with 1 % alcoholic iodine for 10 rnin followed by 5% Na,&O, for 2 min and placed in a n oven at 60 C for 10-30 min to stain in a preheated mixture of 50 ml of ripened Mallory's phosphotungstic acid-hematoxylin and 1 ml of 2% phosphomolybdic acid. Experiments with fixation showed that the stainmg procedure followed Zenker's fluid successfully but not Bouin's fluid. Oxidation by KMnO, was effective only after Zenker fixation; oxidation by CrO, was unsuccessful.Biotech Histochem Downloaded from informahealthcare.com by Cornell University on 12/15/14For personal use only.

I n s t i t u t e f o r R e s e a r c h , P r o d u c t i o n and t a t i c concentration of various compounds r e s d t s i n the enhancement of a c t i v i t y of enzymes catalyzing the transformtion of u r idine t o i t s 5'-phosphates. Partially purified extracts

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