We report on the magnetic resonance spectroscopy (MRS) characterisation of different human meningiomas. Three histological subtypes of meningiomas (meningothelial, fibrous and oncocytic) were analysed both through in vivo and ex vivo MRS experiments. The ex vivo high-resolution magic angle spinning (HR-MAS) investigations, permitting an accurate description of the metabolic profile, are very helpful for the assignment of the resonances in vivo of human meningiomas and for the validation of the quantification procedure of in vivo MR spectra. By using one-and twodimensional experiments, we were able to identify several metabolites in different histological subtypes of meningiomas. Our spectroscopic data confirmed the presence of the typical metabolites of these benign neoplasms and, at the same time, that meningomas with different morphological characteristics have different metabolic profiles, particularly regarding macromolecules and lipids. The ex vivo spectra allowed a better understanding and interpretation of the in vivo MR spectra, showing that the HR-MAS MRS technique could be a complementary method to strongly support the in vivo MR spectroscopy and increase its clinical potentiality.
The present case report was aimed at identifying the molecular profile characteristic of a primitive neuroectodermal tumor (PNET) in a 3-year-old child affected by a lesion localized in the cerebellar region. The histological diagnosis was medulloblastoma. In vivo single voxel 1 H magnetic resonance spectroscopy (MRS) shows high specificity in detecting the main metabolic alterations in the primitive cerebellar lesion; a very high amount of the choline-containing compounds and very low level of creatine derivatives and Nacetylaspartate. Ex vivo high resolution magic angle spinning (HR-MAS) 1 H magnetic resonance spectroscopy, performed at 9.4 Tesla on the neoplastic specimen collected during surgery, allows for the unambiguous identification of several metabolites giving a more in-depth evaluation of the metabolic pattern of the lesion. The ex vivo HR-MAS MR spectra show that the spectral detail is much higher than that obtained in vivo and that, for example, myo-inositol, taurine and phosphorylethanolamine contribute to the in vivo signal at 3.2 ppm, usually attributed to choline-containing compounds. In addition, the spectroscopic data appear to correlate with some morphological features of the medulloblastoma. Consequently, the present study shows that ex vivo HR-MAS 1 H MRS is able to strongly improve the clinical possibility of in vivo MRS and can be used in conjunction with in vivo spectroscopy for clinical purposes.
Treatment of 9-methyladenine (9-MeAd) with cis- [PtL 2 (NO 3 ) 2 ] (1) (L = PMe 2 Ph) in a 2:1 molar ratio generated the bis(adduct) cis-[PtL 2 (9-MeAd) 2 ](NO 3 ) 2 (2), which was isolated and fully characterized by multinuclear ( 1 H, 31 P, 13 C, 195 Pt and 15 N) NMR analysis, which showed that the two nucleobases are selectively coordinated through the N1 atom. Small amounts of a mono(adduct) cis-[PtL 2 (S)(9-MeAd)] 2+ (3) (S = solvent) and of a diplatinated species cis-[L 2 Pt(S){9-MeAd(−H)}PtL 2 ] 3+ (4) are formed in DMSO solution when 9-MeAd is present in smaller quantities than 1. Complex 3 is platinated at N1, with a solvent molecule representing the fourth ligand around the metal center. Complex 4 contains an adenine molecule deprotonated and platinated at N1,N6,N7, with two cis-L 2 Pt units bonded to nitrogen atom N1 and to nitrogen atoms N6 and N7, respectively. With increasing relative concentration of the nucleobase, both complexes 3 and 4 progressively convert into the bis(adduct) 2, the only species detectable in solution when the Ad/Pt molar ratio is 2:1. The trinuclear compound cis-[L 2 Pt{9-MeAd(−H)}] 3 (NO 3 ) 3 (5)
Intact Crocus sativus petals were studied for the first time by high-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) spectroscopy, revealing the presence of kinsenoside (2) and goodyeroside A (3), together with 3-hydroxy-γ-butyrolactone (4). These findings were confirmed by HR-NMR analysis of the ethanol extract of fresh petals and showed that, even though carried out rapidly, partial hydrolysis of glucopyranosyloxybutanolides occurs during extraction. On the other hand, kaempferol 3-O-sophoroside (1), which is "NMR-silent" in intact petals, is present in extracts. These results suggest to evaluate the utilization of saffron petals for phytopharmaceutical and nutraceutical purposes to exploit a waste product of massive production of commercial saffron and point to the application of HR-MAS NMR for monitoring bioactive compounds directly on intact petals, avoiding the extraction procedure and the consequent hydrolysis reaction.
1-Methylthymine (1-MeTy) reacts reversibly with the hydroxo complexes cis-[(PMe 3 ) 2 Pt(µ-OH)] 2 X 2 (X − = NO 3 ; ClO 4 ) in various solvents (S = CH 3 CN, H 2 O, DMSO) to give the thyminate derivatives cis-[(PMe 3 ) 2 Pt{1-MeTy(−H)}(S)]X that have been isolated as pure compounds when S is CH 3 CN. The single-crystal X-ray structure of cis-[(PMe 3 ) 2 Pt{1-MeTy(−H)}(CH 3 CN)]ClO 4 shows that the N(3)-platinated nucleobase acts as a monodentate ligand and a molecule of CH 3 CN completes the coordination sphere of the metal. Crystals of the acetamidine derivative cis-[(PMe 3 ) 2 Pt{1-MeTy(−H)}{CH 3 C(NH)NH 2 }]X were isolated from an acetonitrile solution of this complex, in the presence of small amounts of water, after several months at room temperature, [a]
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