Postactivation potentiation (PAP) describes an initial muscular activation with a submaximal or maximal load intensity that produces acute improvements in muscle power and performance in subsequent explosive activities. The objective of this study was to compare the effect of different PAP protocols in rowing performance. A crossover design involving seven rowers was used, in which two different PAP protocols were applied: PAP of maximal conditioning contractions (PAP MCC) on a rowing ergometer to provide greater transferability and, thus, enhance the magnitude of PAP stimuli on subsequent rowing performance; and PAP of maximal strength contractions (PAP MSC) in half squat and bench pull exercises, similar to the main exercises in rowing strength training, to perform a 20 s “all-out” test simulating a competition start. Student’s t-test was used to compare means of the variables (p < 0.05). Effect size statistics were calculated using Cohen’s d. The PAP MCC protocol resulted in significant differences, with an extremely large effect size in average power output (p = 0.034, d = 0.98) in the first 3 (p = 0.019, d = 1.15) and first 5 (p = 0.036, d = 0.91) strokes. This group also reached a greater number of strokes (p = 0.049, d = 2.29) and strokes per minute (p = 0.046, d = 1.15). PAP with maximal conditioning contractions in rowing warm-up enhanced subsequent rowing sprint and is an advisable strategy to potentiate performance at the start of rowing competitions and sprint regattas.