Background:Cisplatin cures over 80% of testicular germ cell tumours (TGCTs), and nucleotide-excision repair (NER) modifies the sensitivity to cisplatin. We explored the association between NER proteins and their polymorphisms with cisplatin sensitivity (CPS) and overall survival (OS) of patients with non-seminomatous (ns)-TGCTs.Methods:The expression of ERCC1 and XPA and the presence of γH2AX were evaluated in cancer cell lines and in fresh ns-TGCTs. The ERCC1 protein was also determined in ns-TGCTs. The differences between CPS and non-CPS cell lines and patients were analysed by Student's t- or χ2-tests. The differences in OS were analysed using the log-rank test, and the hazard ratios (HRs) were calculated using the Cox model.Results:High ERCC1 expression was observed in the non-CPS cells, and both ERCC1 and γH2AX expressions were augmented after cisplatin treatment. Increased ERCC1 expression was also identified in non-CPS patients. Neither polymorphism was associated with either CPS or OS. The presence of ERCC1 was associated with non-CPS (P=0.05) and adjusted in the prognosis groups. The HR in ERCC1-negative and non-CPS patients was >14.43, and in ERCC1-positive and non-CPS patients the HR was >11.86 (P<0.001).Conclusions:High levels of ERCC1 were associated with non-CPS, suggesting that ERCC1 could be used as a potential indicator of the response to cisplatin and prognosis in ns-TGCTs.
The polymorphism MAD1 1673 G → A affects SAC functionality, increasing the frequency of aneuploid cells. This polymorphism modifies the response to agents that alter the dynamics of microtubules in patients with ovarian cancer.
1139 Background: Central venous catheters (CVC) are cornerstone for the management of cancer patients on chemotherapy, however their use may be associated with venous thrombosis (VTE). The incidence of symptomatic thrombosis varies widely depending on the threshold for ordering diagnostic tests and the population under study. As opposed to lower extremity thrombosis, which is a marker of underlying tumor aggressiveness portending a poor prognosis in cancer patients, it is not clear if CVC-related thrombosis leads to a detriment in patient survival. Objectives: In this retrospective study we address the prognostic implications of symptomatic CVC-and non CVC-related thrombosis. We also evaluate the relevance of thrombotic risk factors reported for cancer-related VTE. Methods: We included 1088 consecutive patients with cancer and CVC followed in the Catheter Clinic at the NCI-Mexico. Of these, only the 506 with breast cancer diagnosis were included in this report. Clinical criteria that prompted the request for a diagnostic doppler ultrasound were dysesthesia, pain, collateral venous circulation and or edema of any extent or duration, affecting the upper extremity, shoulder, or neck. Results: The prevalence of symptomatic CVC-related VTE was 26.1% (132/506), with 61% and 86.7% detected by 60 and 120 days of CVC placement, respectively. The side of catheter placement was not relevant for the development of thrombosis (Right:64/132, Left:68/132). Non-CVC-related upper extremity VTE (33/6.5%) and lower extremity VTE (20/3.9%) were confirmed by doppler ultrasound. Only leucocytosis (>11,000/mm3) prior to thrombosis (but not hemoglobin or platelet count) was more prevalent in individuals with CVC-related VTE, as compared to those without VTE (11.4% vs 4.3%, p=0.006). It is noteworthy that CVC-related upper extremity DVT did not impact overall survival (OS) when compared by bivariate analysis to patients without thrombosis (HR: 0.96, CI 9.75–1.2, p 0.760), with a median survival not reached in either group (18.7% and 18.3% deaths respectively). Non-CVC-related upper extremity VTE may also lack impact on survival (HR:1.16, CI 0.9–1.48, p 0.254), though the number of patients is small. In contrast, the detrimental effect of lower extremity DVT as compared to individuals without thrombosis was significant (HR: 4.6 CI 2.3–9.1 p<.0001, 62.5% deaths). Conclusions: CVC-associated VTE was detected in a fourth of patients primarily during initial treatment. The site of catheter placement did not affect the risk of VTE. Except for leucocytosis prior to thrombosis, other thrombosis risk score parameters useful for lower extremity VTE may not be applicable to CVC-related thrombosis. Importantly, CVC-related thrombosis was not associated with underlying enhanced aggressive tumor behavior as evidenced by a lack of impact on survival. Disclosures: No relevant conflicts of interest to declare.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.