Brain-derived neurotrophic factor (BDNF) is important in supporting neuronal development. BDNF imbalance due to excessive neuronal inhibition can result in the apoptotic degeneration of developing neurons. Since general anesthetics cause profound depression of neuronal activity and are known to induce widespread degeneration in the developing brain, we studied their potential to activate BDNF-mediated developmental neuroapoptosis. When P7 rats (at the peak of brain development) were exposed to a commonly-used and highly pro-apoptotic anesthesia protocol (midazolam, isoflurane, nitrous oxide) for a period of 2, 4 or 6 h, we found that anesthesia modulates the key steps in BDNF-activated apoptotic cascade in two of the most vulnerable brain regions--cerebral cortex and thalamus in time-dependent fashion by activating both Trk-dependent (in thalamus) and Trk-independent p75NTR dependent (in cerebral cortex) neurotrophic pathways. beta-estradiol, a sex hormone that upregulates the protein levels of the activated Akt, protects against anesthesia-induced neuroapoptosis.

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An accurate flow law for dislocation creep of quartz aggregates is critical for the understanding of continental rheology, numerical modeling of lithospheric processes, and the interpretation of microstructures of natural quartz-bearing mylonites. Despite many decades of research, considerable discrepancies still exist among quartz flow laws determined from different experiments. We demonstrate that the key to reconcile these discrepancies is to consider the pressure dependence of the activation enthalpy. From existing high-quality creep experiments on quartz aggregates, we critically identified test runs having microstructures and stress-strain curves indicating steady state regimes 2 and 3 dislocation creep and used the data to obtain a set of flow law parameters most consistent among existing experiments. Because estimates of strain rate and stress from natural mylonites are bound with large uncertainties, they cannot be used to construct a flow law better than one determined from well-controlled creep experiments. A large number of geological studies and modern GPS observations suggest that crustal scale ductile shear zones likely flow at a strain rate range between 10 −13 and 10 −11 s −1 . In this strain rate range, our flow law predicts flow stresses broadly consistent with stress estimates from many natural mylonites based on well-calibrated piezometers for dynamically recrystallized quartz grains.

Cyclin-dependent kinase 1 (Cdk1) kinase dephosphorylation and activation by Cdc25 phosphatase are essential for mitotic entry. Activated Cdk1 phosphorylates Cdc25 and other substrates, further activating Cdc25 to form a positive feedback loop that drives the abrupt G2/mitosis switch. Conversely, mitotic exit requires Cdk1 inactivation and reversal of Cdk1 substrate phosphorylation. This dephosphorylation is mediated, in part, by Clp1/Cdc14, a Cdk1-antagonizing phosphatase, which reverses Cdk1 phosphorylation of itself, Cdc25, and other Cdk1 substrates. Thus, Cdc25 phosphoregulation is essential for proper G2-M transition, and its contributions to cell cycle control have been modeled based on studies using Xenopus and human cell extracts. Because cell extract systems only approximate in vivo conditions where proteins interact within dynamic cellular environments, here, we use Schizosaccharomyces pombe to characterize, both experimentally and mathematically, the in vivo contributions of Cdk1-mediated phosphorylation of Cdc25 to the mitotic transition. Through comprehensive mapping of Cdk1 phosphosites on Cdc25 and characterization of phosphomutants, we show that Cdc25 hyperphosphorylation by Cdk1 governs Cdc25 catalytic activation, the precision of mitotic entry, and unvarying cell length but not Cdc25 localization or abundance. We propose a mathematical model that explains Cdc25 regulation by Cdk1 through a distributive and disordered phosphorylation mechanism that ultrasensitively activates Cdc25. We also show that Clp1/Cdc14 dephosphorylation of Cdk1 sites on Cdc25 controls the proper timing of cell division, a mechanism that is likely due to the double negative feedback loop between Clp1/Cdc14 and Cdc25 that controls the abruptness of the mitotic exit switch. mitotic bistability | multisite phosphorylation

Mast cells, critical mediators of inflammation and anaphylaxis, are poised as one of the first lines of defense against external assault. Mast cells release several classes of preformed and de novo synthesized mediators. Cross-linking of the high-affinity FcΕRI results in degranulation and the release of preformed, proinflammatory mediators including histamine and serotonin. We previously demonstrated that mast cell activation by Listeria monocytogenes requires the α2β1 integrin for rapid IL-6 secretion both in vivo and in vitro. However, the mechanism of IL-6 release is unknown. Here, we demonstrate the Listeria- and α2β1 integrin-mediated mast cell release of preformed IL-6 without the concomitant release of histamine or β-hexosaminidase. α2β1 integrin-dependent mast cell activation and IL-6 release is calcium independent. In contrast, IgE cross-linking-mediated degranulation is calcium dependent and does not result in IL-6 release, demonstrating that distinct stimuli result in the release of specific mediator pools. These studies demonstrate that IL-6 is presynthesized and stored in connective tissue mast cells and can be released from mast cells in response to distinct, α2β1 integrin-dependent stimulation, providing the host with a specific innate immune response without stimulating an allergic reaction.

No abstract

Phosphorylation of the kinetochore component Nsk1 by Cdk1 antagonizes its localization to and function at the kinetochore and spindle during early mitosis.

Rocks with a well‐developed lineation but weak or no foliation (L‐tectonites) commonly occur as isolated volumes dispersed in other tectonites. We consider L‐tectonites that reflect constrictional finite strains here and use a multiscale approach to investigate the conditions for constrictional strain fields. The approach combines the strength of kinematic and mechanical analyses in large strain three‐dimensional deformations. Our modeling shows that, in simple shearing and thinning zone progressive deformations, constrictional strains develop only in rheological heterogeneities that are moderately stronger than the bulk material as a whole. Stronger elements never accumulate enough internal strains for any fabric to develop. Inclusions weaker than the bulk material will develop flattening strains. L‐tectonites are most likely developed in macroscale simple shearing, simple‐shearing‐dominated plane‐strain general shearing, or simple‐shearing‐dominated Sanderson and Marchini transpression. The lineations of the L‐tectonites are always nearly parallel to the lineations in the bulk material. Where the lineations are nearly 90° from the vorticity axis, the macroscale flow is close to a plane‐strain general shearing. Where the lineations are oblique to the vorticity axis or more variable, a simple‐shearing‐dominated triclinic thinning zone with mainly uniaxial boundary stretching is likely. The concept of homogeneous transtension deformation, combining a homogeneous pure shearing and a transcurrent simple shearing, is unsupported by fabric evidence and is likely unrealistic. Under an oblique divergence boundary condition, the upper lithosphere deforms by folding and fracturing and the ductile lithosphere develops simple‐shearing‐dominated detachment shear zones. Constrictional strains (hence L‐tectonites) can develop in these detachment zones due to flow partitioning.