(i) Psychological distress was relatively frequent in our patients with psoriasis; (ii) female gender was the most important predictive factor for psychological distress; (iii) there was no association between psoriasis severity and psychological distress; (iv) planning and active coping were the coping strategies most frequently employed by patients; and (v) most dermatologists employed a problem-orientated attitude in caring for patients.

Objectives: To use a digital dermoscopy analyzer with a series of "borderline" pigmentary skin lesions (ie, clinically atypical nevi and early melanoma) to find correlation between the studied variables and to determine their discriminating power with respect to histological diagnosis.Design: A total of 147 pigmentary skin lesions were histologically examined by 3 experienced dermatopathologists and identified as nevi (n = 90) and melanomas (n = 57). The system evaluated 36 variables to be studied as possible discriminant variables, grouped into 4 categories: geometries, colors, textures, and islands of color.Setting: University medical department.Patients: A sample of patients with excised pigmen-tary skin lesions (nevi and melanomas).Main Outcome Measures: Sensitivity, specificity, and accuracy of the model for evaluating "borderline" pigmentary skin lesions.Results: After multivariate stepwise discriminant analysis, only 13 variables were selected to compute the canonical discriminant function. Conclusion:The present method made it possible to determine which objective variables are important for distinguishing atypical benign pigmentary skin lesions and early melanoma.

Noninvasive diagnostic methods such as dermoscopy or epiluminescence light microscopy have been developed in an attempt to improve diagnostic accuracy of pigmented skin lesions. The evaluation of the many morphologic characteristics of pigmented skin lesions observable by epiluminescence light microscopy, however, is often extremely complex and subjective. With the aim of obviating these problems of qualitative interpretation, methods based on mathematical analysis of pigmented skin lesions have recently been designed. These methods are based on computerized analysis of digital images obtained by epiluminescence light microscopy. In this study we used a digital dermoscopy analyzer with 147 clinically atypical pigmented skin lesions (90 nevi and 57 melanomas) to determine its discriminating power with respect to histologic diagnosis. The system evaluated 48 objective parameters used to train an artificial neural network. Using the artificial neural network with 10 variables selected by a stepwise procedure, we obtained a maximum accuracy in distinguishing melanoma from benign lesions of about 93%. Comparing this result with those of the many studies using classical epiluminescence light microscopy, it emerges that the method proposed is equal or even superior in diagnostic accuracy and has the advantage of not depending on the expertise of the clinician who examines the lesion.

SUMMARYExtracorporeal photochemotherapy ( ECP) has been shown to be a potent activator of peripheral blood macrophages because it causes a marked release of macrophage-dependent proinflammatory cytokines, and it is therefore currently considered to be a safe and non-toxic immunomodulatory treatment. On this basis we studied the function of peripheral blood mononuclear cells (PBMC ) in eight patients with early stage (Ib) cutaneous T-cell lymphoma (CTCL), before and 1 year after ECP, together with their clinical and histological responses. In particular we evaluated in vitro phytohaemagglutinin (PHA)-stimulated proliferation and production of interleukin-4 (IL-4) and interferon-c (IFN-c) as well as lipopolysaccharide (LPS)-induced production of IL-12. Before treatment we observed that PBMC of patients produced significantly higher levels of IL-4 and lower levels of IFN-c and IL-12 than those of healthy control subjects. After 1 year of ECP, IL-4, IFN-c and IL-12 production no longer differed from that of control subjects. Moreover, we observed a good clinical result matched by histological response. Our data confirm that earlystage CTCL patients show a predominantly type-2 immune response that might be responsible for several immunological abnormalities found in this disease. We have demonstrated that ECP reverses the T-helper type 1/T-helper type 2 (Th1/Th2) imbalance and may therefore be considered an efficient biological response modifier.