The aim of this study was to determine the relationship between pilot workload, performance, subjective fatigue, sleep duration, number of sectors and flight duration during short-haul operations. Ninety pilots completed a NASA Task Load Index, Psychomotor Vigilance Task and a Samn-Perelli fatigue scale on top-of-descent of each flight and wore an activity monitor throughout the study. Weak, but significant, correlations were revealed between workload and all factors. Subjective fatigue, number of sectors and lapses were significant predictors of workload. Pilots reported higher workload when fatigue increased, the number of sectors were higher, and objective performance was worse.