-The objective of this work was to evaluate if corn plants damaged by the lesser cornstalk borer (Elasmopalpus lignosellus) larvae release volatile organic compounds capable of attracting the egg parasitoid Trichogramma pretiosum. The treatments consisted of plants subjected to harm caused by E. lignosellus larvae, plants subjected to mechanical damage, and undamaged plants. The parasitoid was more attracted by the volatiles released by the insect damaged plants than to those released by undamaged corn plants, after 24 and 72 hours. The volatiles (Z)-3-hexenyl acetate, β-pinene, β-myrcene, (E)-4,8-dimethylnona-1,3,7-triene, and benzothiazole were released in significantly larger quantities by damaged plants. Volatiles released by corn plants damaged by E. lignosellus larvae may act as an indirect defense, attracting by T. pretiosum.Index terms: egg parasitoid, tritrophic interaction, semiochemicals, volatile organic compounds.
Atração de Trichogramma pretiosum devida ao dano causado porElasmopalpus lignosellus em milho
The objective of this work was to evaluate the reproductive behavior and response of Elasmopalpus lignosellus (Zeller) males to calling females. Frequency of mating was recorded in couples during the first 7 days of the adult stage. Calling behavior of females was observed during the first 4 days of the adult stage and responses of males, in the same age intervals, to calling females were recorded in wind tunnel bioassays. The maximum number of matings occurred when the couple was between 24 and 48 h old. The scotophase period significantly influenced mating behavior, which peaked between 6 and 8 h of darkness and the mean mating duration was 93.9 ± 4.2 min. Calling females, when evaluated in a wind tunnel, attracted significantly more males than in bioassays with clean air (control). The number of individuals in calling behavior was significantly lower for females that were between 0 to 24 h old compared to the other females evaluated, but this did not influence male response. A lower proportion of males between 48 to 72 h old responded to calling females and these responses were delayed in comparison with males of other ages (0 to 24, 24 to 48, and 72 to 96 h old). These results indicate that the age of E. lignosellus males influences the response to conspecific calling females.
Objetivou-se neste trabalho avaliar a patogenicidade de isolados do fungo Metarhizium anisopliae em Scaptocoris carvalhoi BECKER, 1967, bem como determinar a Dose Letal média (DL50) e o Tempo Letal médio (TL50), em laboratório. Os experimentos foram conduzidos na Embrapa Agropecuária Oeste em Dourados, MS, durante 2003. Suspensões de quatro isolados de M. anisopliae (Ma7, Ma69, Ma283 e Ma342) foram preparadas nas concentrações 10(4), 10(5), 10(6), 10(7) e 10(8) conídios mL-1 e inoculados topicamente 5mil dessas suspensoes sobre S. carvalhoi correspondendo, respectivamente, a 50, 500, 5.000, 50.000 e 500.000 conídios percevejo-1. Após a inoculação, os insetos (10 adultos e cinco ninfas) foram acondicionados em gerbox (parcela) e mantidos em câmaras climatizadas reguladas para 26±1ºC, UR 85%, sem fotofase. Na maior dose testada (500.000 conídios inseto-1), observou-se um incremento significativo de mortalidade do percevejo com o aumento da dose do fungo, para todos os isolados testados. Os menores valores da DL50 foram observados com os isolados Ma69 e Ma7 e o maior com Ma283. Os valores do TL50 variaram de 0,32 a 5,84 dias, sem diferirem significativamente, entre si. Os isolados Ma69 e Ma7 apresentam potencial para serem empregados no controle de S. carvalhoi a campo.
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