The objective of this study was to optimize the conditions for extracting bioactive compounds from barbatimão barks. Two levels and three variables were applied to a factorial design 2 3. The independent variables were extraction temperature (30-50 °C), solution pH (7-11) and sample size (0.250-0.841 mm), while the variables the answer was the content of total polyphenols, condensed tannins and antioxidant activity. Experimental results were fitted to a first order linear polynomial model. Analysis of variance was used to determine the optimal conditions for the maximum content of total polyphenols, condensed tannins and antioxidant activity. Optimized conditions were with extraction temperature of 48 °C, pH of solution 7 and sample size of 0.841 mm. Under these conditions, the maximum values predicted were 12.76 g of gallic acid equivalent / 100 g of bark for total polyphenols, 27.91 g of catechin equivalent / 100 g of bark for condensed tannins, and 0.439 g of equivalent trolox / 100 g of bark for antioxidant activity. The extract obtained experimentally was close to the predicted values, promoting an extraction of compounds with high levels of phenolic compounds and antioxidant capacity.
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