In search for a more sustainable agriculture, the use of microorganisms as a technology is increasingly being used by agriculture throughout the world. This is due to the fact that it minimizes the use of agricultural supplies reducing environmental costs and impacts, based on the beneficial and natural relationships between edaphic organisms and cultivated plants. The rhizobacteria habitat in the soil establishes biochemical relationships with the plants acting as plant growth promoters (PGPR). Many of these bacteria are producers of phytohormones and enzymatic compounds with the capacity to provide important nutrients for plants. In this context, the present work aimed to quantify the potential of indole-3-acetic acid (IAA) production and the phosphate solubilization of rhizobacteria from Western Paraná. Isolates grown in DYGS medium plus tryptophan were quantified by colorimetry for the production of IAA. Iron phosphate solubilization was carried out by inoculation in modified Pikovskaya medium (PKV) and quantified by colorimetry. The results were evaluated by the Scott-Knott test at 5% using the SASM-Agri program. The highest IAA production was observed with the addition of tryptophan to Erwinia (219); Enterobacter (302) and Salmonella (57). Isolates Falsibacillus (438) and 505 showed higher efficiency in the iron phosphate solubilization. Isolates Enterobacter (130), 438 and Enterobacter (151) were highlighted in both tests, being characterized as a great potential for use in biotechnological products.
This research sought to study the effect of applying swine wastewater (SW) on the microbiological attributes of a clayey soil cultivated with physic nut (Jatropha curcas L.). This experimental area was subjected to SW applications for 3 years, followed by fallow for other 2 years. In December 2017, soil collections were carried out and the experiment conducted in randomized complete blocks (RCB) with six treatments and three replicates, namely T1: 0 (control), T2: 40, T3: 80, T4: 120, T5: 160 and T6: 200 m 3 ha-1. The evaluated attributes were the following: CFU, diversity, MBC, SBR, qCO 2 and FDA. Values of the CFU means demonstrated an increase in the bacterial population density and the diversity pointed to the favoring of bacterial morphotypes already present in the soil, in all treatments under SW application. CFU and SBR were the most effective variables in obtaining results and in the treatment discrimination. The dose with the best metabolic performance was 160 m 3 ha-1 due to the better relationship of the parameters previously evaluated in this study.
The objective of this study was to investigate28 strains of nativerhizobacteriafrom the west region of Paraná, evaluating the acetoin and chitinase production capacity, because of their roleinbiocontrol. Thebacteriaisolates were submitted to the modified Voges-Proskauer (VP) assayin Clark & Lubs liquid medium forglucose fermentation. Metaboliteproductionwasevaluated in a spectrophotometer,and it showed a wide variation in concentrations (0,476to1,865) for differentisolates.Strains 241, 320, 326, and 273showedhighervaluesthan the others tested. According to positive results in the qualitative test of VP, 11 isolates were chosen for the tests in minimal medium containing chitin as the only source of carbon. Isolates that presented the degradation halo formation were considered as chitinolytic bacteria, evidencing chitinaseactivity. Among these strains, two belonged tothe genus Bacillusspp. (56 and 121) and two tothe genus Enterobacterspp. (292 and 151). Therefore, the present work was able to identify native strains with potential for biocontrol studies allowing tocontinue investigations of other metabolitesproducedby thosebacteria in this collection regarding plant protection.
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