Tityus serrulatus is a Brazilian scorpion species with great medical significance. While the effects of neurotoxins have been extensively studied, little is known about the proteases expressed in the venom gland of this arthropod. In this study, clones from a T. serrulatus (Ts) venom gland cDNA library were selected according to homology to proteases. The sequences were aligned in the database and classified by homology. Similarity and identity analyses of the sequences were carried out, and a phylogenetic tree was constructed with the sequences of other proteases. These cDNA sequences correspond to ten different metalloproteases, named metalloserrulases (TsMS). TsMS 1-9 belong to the metzincin family, which has three domains: signal peptide, propeptide, and metalloprotease domain; while TsMS 10 belongs to the gluzincin family. The proteolytic activity of the venom was inferred from the cleavage of fibrinogen, and the residues recognized by the proteases were determined by cleavage of a tripeptide library using a fluorescence resonance energy transfer assay. The Ts venom showed proteolytic activity on fibrinogen and preferential cleavage close to the basic residues K and R. Its activity could be inhibited by EDTA, indicating that the venom from this scorpion predominantly consists of metalloproteases.

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This work presents an integrated pixel topology that promises to offer superior performance in a Hartmann-Shack Wavefront Sensor (WFS) with an orthogonal array of Quad Cells serving as Position-Sensitive Detectors. The readout integrated circuit for each photodiode is fully compliant to any standard CMOS microelectronics technology and is advantageously tolerant to high background illumination levels whereas maintaining both high linearity and high sensitivity. To assess the operation of this pixel on the focal-plane array of the WFS, we developed a computational platform encompassing a full detection chain comprising wavefront sampling, photodetection, electronic circuitry and wavefront reconstruction. It couples an algorithm written in C to SPICE (Simulated Program with Integrated Circuits Emphasis). The platform is technology agnostic and flexible, enabling easy modification to represent different detection or reconstruction methods. The results obtained with the proposed pixel have been compared to those obtained with a conventional pixel in CMOS image sensor.

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