(1) Background: The amino acid arginine is now receiving great attention due to its potential anti-caries benefits. The purpose of this in vitro study was to evaluate the shear bond strength (SBS), ultimate tensile strength (UTS), and antimicrobial potential (CFU) of two arginine-containing orthodontic resin cements. (2) Methods: Forty bovine incisors were separated into four groups (n = 10): Orthocem, Orthocem + arginine (2.5 wt%), Transbond XT, and Transbond XT + arginine (2.5 wt%). The brackets were fixed to the flat surface of the enamel, and after 24 h the SBS was evaluated using the universal testing machine (Instron). For the UTS test, hourglass samples (n = 10) were made and tested in a mini-testing machine (OM-100, Odeme). For the antibacterial test (colony forming unit-CFU), six cement discs from each group were made and exposed to Streptococcus mutans UA159 biofilm for 7 days. The microbiological experiment was performed by serial and triplicate dilutions. The data from each test were statistically analyzed using a two-way ANOVA, followed by Tukey’s test (α = 0.05). (3) Results: The enamel SBS mean values of Transbond XT were statistically higher than those of Orthocem, both with and without arginine (p = 0.02033). There was no significant difference in the SBS mean values between the orthodontic resin cements, either with or without arginine (p = 0.29869). The UTS of the Transbond XT was statistically higher than the Orthocem, but the addition of arginine at 2.5 wt% did not influence the UTS for either resin cement. The Orthocem + arginine orthodontic resin cement was able to significantly reduce S. mutans growth, but no difference was observed for the Transbond XT (p = 0.03439). (4) Conclusion: The incorporation of arginine to commercial orthodontic resin cements may be an efficient preventive strategy to reduce bacterial growth without compromising their adhesive and mechanical properties.
O ensino de fisiologia do crescimento e desenvolvimento humano visa avaliar aspectos da reprodução e crescimento humano, enfocando o planejamento da concepção, gravidez, infância, adolescência, fase adulta e senilidade, focando o entendimento dos mecanismos específicos pela regulação e controle dos sistemas e órgãos, principalmente o sistema endócrino, responsáveis por coordenação e ajustes que permitem a viabilidade do organismo. O curso é composto de aulas teóricas e aplicação/execução de aulas praticas. As aulas praticas correspondentes às aulas teóricas foram elaboradas pelos grupos de alunos matriculados na disciplina BF590 - Fisiologia Humana do Crescimento e Desenvolvimento. Assim, o proposto foi instigar a curiosidade e a imaginação dos alunos, com a iniciativa de uso de métodos alternativos diversos, com o intuito de não utilização de animais, para que os mesmos propusessem aulas práticas que correspondessem ao tema de aula ministrado e a efetivação das mesmas pelos próprios alunos com a supervisão do professor.
Sugarcane (Saccharum spp.) is one of the most well-known plants which possesses a large accumulation of sucrose. Another cultivar, energy cane, is an interspecific hybrid with higher fiber and lower sugar content than sugarcane. Commercial cultivation of sugarcane and energy cane is carried out by vegetative propagation, through the distribution of culm segments (setts) or pre-sprouted seedlings (PSS). In this context, the metabolism of axillary bud outgrowth is crucial for cultures that use vegetative propagation. In this work, we evaluate the metabolic profile of sugarcane and energy cane in the early hours during the axillary bud outgrowth. Sugarcane showed few metabolic changes, except for the significant increase in glutamate levels, which may be associated with root formation in the culm. In contrast, energy cane presented significant changes in amino acid catabolism, increased levels of reducing sugars, lipids, and metabolite activity in the phenylpropanoid pathway. These results together reveal changes in the energy and redox status of the cell, electron transport for the TCA cycle, and an increase in compounds related to cell wall formation and growth in energy cane. Our study provides new insights on the regulation of the axillary bud of species of the Saccharum complex.
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