The vasoactive proline-rich oligopeptide termed BPP-BrachyNH (H-WPPPKVSP-NH) induces in vitro inhibitory activity of angiotensin I-converting enzyme (ACE) in rat blood serum. In the present study, the removal of N-terminal tryptophan or C-terminal proline from BPP-BrachyNH was investigated in order to predict which structural components are important or required for interaction with ACE. Furthermore, the toxicological profile was assessed by in silico prediction and in vitro MTT assay. Two BPP-BrachyNH analogues (des-Trp-BPP-BrachyNH and des-Pro-BPP-BrachyNH) were synthesized, and in vitro and in silico ACE inhibitory activity and toxicological profile were assessed. The des-Trp-BPP-BrachyNH and des-Pro-BPP-BrachyNH were respectively 3.2- and 29.5-fold less active than the BPP-BrachyNH-induced ACE inhibitory activity. Molecular Dynamic and Molecular Mechanics Poisson-Boltzmann Surface Area simulations (MM-PBSA) demonstrated that the ACE/BBP-BrachyNH complex showed lower binding and van der Wall energies than the ACE/des-Pro-BPP-BrachyNH complex, therefore having better stability. The removal of the N-terminal tryptophan increased the in silico predicted toxicological effects and cytotoxicity when compared with BPP-BrachyNH or des-Pro-BPP-BrachyNH. Otherwise, des-Pro-BPP-BrachyNH was 190-fold less cytotoxic than BPP-BrachyNH. Thus, the removal of C-terminal proline residue was able to markedly decrease both the BPP-BrachyNH-induced ACE inhibitory and cytotoxic effects assessed by in vitro and in silico approaches. In conclusion, the aminoacid sequence of BPP-BrachyNH is essential for its ACE inhibitory activity and associated with an acceptable toxicological profile. The perspective of the interactions of BPP-BrachyNH with ACE found in the present study can be used for development of drugs with differential therapeutic profile than current ACE inhibitors.
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