Background: This study sought to determine the dynamics of IL gene expression during serous membrane damage using an animal model of aseptic peritoneal injury.Methods: In our study, we used 35 male Wistar rats. Macroscopic and microscopic studies were conducted between 6 hours and 30 days after peritoneal damage was induced. In the damaged peritoneal area, we assessed IL gene expression across the experimental timeframe.Results: We found that the majority of the studied genes had three characteristic peaks in expression: at 6 hours, on day 3, and on day 14. These effects were observed for chemokine (CXC motif) ligands 1 and 3, IL1b, and IL6. Two peaks of increased expression (on days 3 and 14) were noted for CXCL1, CXCL5, INFγ, IL2, IL4, IL10, TNF, and CD40LG.Conclusion: We hypothesize that the absence of attention to the changes that occur in the peritoneum after aseptic damage has prevented research from focusing on the important stage of the formation of the richly vascularized adhesions that are unable to regress. Based on the results of our study, we conclude that it is critically important to influence the last wave of IL expression activation (2 weeks after aseptic peritoneum damage) to effectively prevent adhesion formation.(International Journal of Biomedicine. 2017;7(4):293-297.)
ObjectivesTo study the expression of growth factors in the regulation of tissue repair after peritoneal damage tissue response to peritoneal damage.MethodsExperimental study in 35 male Wistar rats determining the evolution over time of the tissue response to aseptic peritoneal damage. A standardized bowel and peritoneal lesions were created in the right lower quadrant by laparotomy. Then, tissular expression of growth factors was evaluated by multiplex polymerase chain reaction at seven timepoints between 6 h and 30 days, postoperatively.ResultsTissular responses of granulocyte-stimulating factors (Csf2, Csf3), connective tissue growth factor (Ctgf), epidermal growth factors and receptor (Egf, Egfr), fibroblast growth factors (Fgf2, 7 and 10), heparin binding EGF-like growth factor (Hbegf), hepatocyte growth factor (Hgf), insulin-like growth factor-1 (Igf1), mitogenic transforming growth factors (Tgfa, Tgfb1, Tgfbr3), and vascular endothelial growth factor A (Vegfa) were biphasic with a first expression peak at day 3, followed by a more pronounced peak at day 14.ConclusionsWe observed a long-lasting, widespread response of tissular growth factors for at least two weeks after peritoneal damage. To be clinically effective, the prophylaxis of postoperative adhesions might be needed for an extended period of time.
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