Europe’s prehistory oversaw dynamic and complex interactions of diverse societies, hitherto unexplored at detailed regional scales. Studying 271 human genomes dated ~4900 to 1600 BCE from the European heartland, Bohemia, we reveal unprecedented genetic changes and social processes. Major migrations preceded the arrival of “steppe” ancestry, and at ~2800 BCE, three genetically and culturally differentiated groups coexisted. Corded Ware appeared by 2900 BCE, were initially genetically diverse, did not derive all steppe ancestry from known Yamnaya, and assimilated females of diverse backgrounds. Both Corded Ware and Bell Beaker groups underwent dynamic changes, involving sharp reductions and complete replacements of Y-chromosomal diversity at ~2600 and ~2400 BCE, respectively, the latter accompanied by increased Neolithic-like ancestry. The Bronze Age saw new social organization emerge amid a ≥40% population turnover.

Monumental enclosures are a widespread phenomenon of the European Neolithic. One category of enclosure is the mid-fifth-millennium BC rondel sites of Central Europe. In parts of this region, rondel sites are grouped, drawing attention to notable differences in individual rondel forms. Here, we use Bayesian modelling of radiocarbon dates from the ditches of two rondels at Praha-Krč, Bohemia, to demonstrate their contemporaneity. In turn, this informs interpretations of the role played by multi-rondel sites in symbolic competition between regional communities, who invested in rondels as part of translocal negotiation. The concept of translocality may prove fruitful for the investigation of the monumental architecture of other periods and regions.