Objective This research aims to evaluate the preclinical meritorious and anticancer effects of Metformin in a Xenograft model of breast cancer. Methods This interventional trial was conducted during a defined period of 5 months (August 2016 January 2017). We used a Xenograft model of nude BALB/c mice. A sample size of 50 mice, allocated into two groups and designated as Group A and Group B for Metformin and negative control groups, respectively. The anticancer activity of Metformin has been evaluated by comparing the tumour volume, tumour weight, tumour regression ratio, percentage regression, and survival rate. Results Compared with the control group, Metformin can significantly reduce the progression of tumour in the Xenograft model of breast cancer induced by MCF-7. This is reflected by significant differences in tumour volume at the final follow-up ( p = <0.001). Our findings are further supported by a significant reduction of the tumour growth rate ( p = <0.001) and tumour weight ( p = <0.001) in the Metformin group than in the control group. Similarly, the total survival rate and tumour regression are more significantly correlated in the Metformin group. Conclusion This study demonstrates that Metformin can significantly reduce the tumour growth and can increase the survival rate in a Xenograft model of breast cancer.
Objective: To assess the role of radiological predictive markers on orthopantomogram for inferior alveolar nerve (IAN) injury related to the removal of mandibular third molar surgery and the occurrence of postoperative IAN paresthesia. Material and Methods: This prospective observational study was conducted on 60 patients (aged 17-35 years) indicated for extraction and showed one or more of the seven previously known panoramic radiographic risk signs of IAN injury. Variables such as age, sex, tooth angulation, and relationship with the inferior alveolar canal (IAC) were assessed to see their outcome on IAN injury. Data analysis is presented through tables and descriptive methods. Results: Among patients, 26 were male and 34 were female, with a mean age of 26.17 years. Out of seven radiological predictive markers, only six were found in this study, whereas one marker, viz. interruption of white line of the canal was not found. After surgical removal of the lower third molar, only two patients with radiographic signs showing the deflection of roots and darkening of roots continued with sensory deficit 5 weeks post-operatively. Conclusion: The risk of inferior alveolar nerve injury during lower third molar surgery is very low, even in patients with radiological predictive markers.
Aim: To look at anti-cancer effects of a variety of novel drugs on a variety of cancerous cell lines in the quest for a more inexpensive and effective anti-cancer treatment. Methodology: The research was carried out in conjunction with PCMD at JPMC's BMSI Department of Pharmacology. The experiment lasted about eight months (from April to November 2016). Noninvasive estrogen-dependent tumours, invasive estrogen-independent breast cancer, colorectal cancer, and cervical cancer were all depicted using MCF-7, HT-29, MDA-MB-231and HeLa cell lines, in that order. The MTT assay was used to improve the drugs' anticancer or antiproliferative efficacy in vitro. Using the MTT test, we determined the vitality of all treated tumour cell lines as well as the IC50 values of each chemical against all malignant cell lines. Results: This study demonstrated thatMetformin significantly decreased the survivability of MCF7, HT-29, MDA-MB-231, and Hela cell lines when compared to Apricoxib and Mebendazole. As a result, comparing the IC50 values of the studied agents for each of the evaluated treated cells backed up this claim. Hence, for Hela(p=0.386), MCF-7 (p=0.083), and MDA-MB-231 (p=0.083) cell lines but pochoc analysis revealed no significant differences in IC50 values of Metformin and Methotrexate. Conclusion: When compared to Apricoxib and Mebendazole, Metformin has a significant effect on the survivability of the cell lines studied. Metformin, as a result, would have been a wonderful chemotherapeutic addition Keywords: Cell lines, In vitro, MCF-7, Hela, MDA-MB-231
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