A flow injection method with chemiluminescence detection is reported for the determination of vitamin A. The method is based on the enhancement effect of vitamin A on chemiluminescence of tris(2,2'-bipyridyl)Ru(II)-Ce(IV) in acidic medium. The proposed procedure is used to quantitate vitamin A in the range 1.0-100 x 10(-6) mol/L with a correlation coefficient of 0.9991 (n = 9) and relative standard deviation in the range 1.2-2.3% (n = 4). The limit of detection (3 x blank) was 8.0 x 10(-8) mol/L( )with a sample throughput of 100/h. The effect of common excipients used in pharmaceutical formulations and some clinically important compounds was also studied. The method was applied to determine vitamin A in pharmaceutical formulations and the results obtained were in reasonable agreement with the amount quoted. The results were compared using spectrophotometric method and no significant difference was found between the results of the two methods at 95% confidence limit.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.