Ficus palmata (F. palmata) is a medicinally very rich plant that is used as a folk medicine to treat many diseases. This study investigated the anticancer effect of 70% ethanolic leaves extract of F. palmata alone and its combinational study with photosense-mediated photodynamic therapy (PDT) and doxorubicin hydrochloride (Dox-HCl) on rhabdomyosarcoma (RD) cells. The inhibitory dose (IC50) was calculated from dose response analysis of 24 h post exposure of F. palmata (400, 200, 100, 50, 25, 12.5 µg ml−1), Dox-HCl (4, 3, 2, 1 µM), and photosense (80, 40, 20, 10, 5 µM, 10 J cm−2) mediated PDT. These IC50 doses were further used to assess the anticancer effect of F. palmata in combination with photosense-mediated PDT and Dox-HCl. The in vitro findings showed that the 70% ethanolic F. palmata leaves extract has anticancer potential, and has the ability to enhance the anticancer effect in combination with other therapies. Moreover, the neoadjuvent combination of F. palmata with PDT and chemotherapy has a synergistic effect. This study provides evidence that F. palmate leaves extract possesses promising anticancer activity, and more research is needed to isolate the specific bioactive compound for cancer treatment.
Phytocompounds play a key role in prevention and treatment of cancers. The secondary metabolites are being studied due to their anticancer and antioxidant properties leading to the finding of new therapeutic agents. Like many medicinal plants, Ficus racemosa (FR) is rich in polyphenols and can be used in cancer treatment. The side effects of existing therapeutic modalities at high doses may be reduced in combination. In this study the anticancer and anti-migration potentials of FR were evaluated, along with its combinational study at low therapeutic dosage with doxorubicin-HCl (Dox-HCl), dacarbazine (DTIC) and photodynamic therapy (PDT). The effects of FR leaves extract on the rhabdomyosarcoma (RD) cells morphology, cell viability, and cell migration ability were investigated by light microscopy, MTT assay, and wound healing cell migration assay respectively. The effect of various FR concentrations on RD cells indicated the potential anticancer effect in a dose dependent manner. The results of low dose di-combinations (FR-chemo, FR-PDT) and tri-combinations (FR-chemo-PDT) were synergistic. The % cell viability was reduced to 29% for FR-Dox-HCl-PDT and 44% for FR-DTIC-PDT combinations. A pronounced cytotoxic effect and cell migration was observed in tri-combination therapy as compared to di- and mono-therapy. This study concluded that FR leaves extract has anticancer effects and gives synergy with chemo and PDT combinations.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.