Mangabeira is a native fruit tree from Brazil, which provides a delicious fruit to taste and beneficial to health, whose information about the seedlings production will assist the entire productive chain of this important species. The aim of this research was to evaluate protected environments and substrates compositions in the formation of mangabeira seedlings. For this, two greenhouses with different screens were used, the first covered with aluminized thermo-reflective screen of 50% shading and the second with black screen of 50% shading. Inside the environments, were tested substrates derived from combinations of various proportions of bovine manure (M), soil (S), medium vermiculite (MV), super fine vermiculite (FV) and fine sand (FS). For each environment of cultivation was adopted a completely randomized design to evaluate the substrates, with five replicates of eight plants. The environments were compared by analysis of experiments groups. The results showed that both protected environments are suitable for mangabeira seedlings. In the aluminized screen is indicated for the formation of seedling in the substrate with 20% M + 30% S + 10% MV + 30% FV + 10% FS while in the black screen is indicated for seedlings in the substrate with 10% M + 30% S + 10% MV + 10% FV + 40% FS and with 10% M + 30% S + 40% MV + 10% FV + 10% FS.