Overall insights in this paper would mean that, while research-based corporations are to be praised whenever waiving, on humanitarian purposes, part of their profits, the trade and profit rules cannot basically be given up if long-term sustainable results are the goal to look for. Only negotiated agreements securing all contracting parties lasting advantages may ensure shifting of such a goal from mere vision to a really sustainable attainment.

A quick and high-through LC-MS/MS method has been developed and applied to simultaneously quantify lamivudine (3TC), stavudine(d4T), zidovudine(AZT), efavirenz(EFV), nevirapine(NVP), and Lopinavir/Ritonavir (LPV/RTV) plasma concentrations. A combination of protein precipitation and liquid-liquid extraction was used to extract all compounds. The method showed a good linearity in a concentration range of 20-3200μg/L for 3TC, d4T, AZT, 40-6400μg/L for EFV and NVP, 62.5-10000μg/L for LPV and 12.5-2000μg/L for RTV. Mean intra-and inter-day precision were within ±20% at the LLOQ and ±15% at the other QC level. The accuracy were between 85% and 115% for all seven analytes. The whole run is 13 minutes. This method has been successfully used for analyzing 133 samples from 84 HIV-positive patients being treated with the combination therapy in China. To our knowledge, it is the fi rst time to use LC-MS/MS method to simultaneously quantify 3TC, d4T, AZT, EFV, NVP, LPV and RTV in China for investigating the relationship between blood drug concentrations and effi cacy and/or toxicity. Material and Methods Chemicals Working standard of lamivudine (3TC), stavudine (d4T), zidovudine (AZT), efavirenz (EFV), nevirapine (NVP), lopinavir (LPV), ritonavir (RTV) and telmisartan (internal standard, IS) were supplied by U.S Pharmacopeia. HPLC grade methanol and acetonitrile were purchased from Merck Ltd., Germany. Analytical reagent (AR) grade ammonium acetate and glacial acetic acid were purchased from Sinopharm chemical Ltd., China. Blank human blood was collected in the tubes containing Ethylene Diamine Tetra acetic Acid (EDTA) from healthy and drug free volunteers. After centrifugation at 4000 rpm at room temperature, plasma was collected and stored at −20°C. LC-MS/MS conditions An HPLC system (Shimadzu, Kyoto, Japan) consisting of a binary LC-20AD prominence pump, autosampler (SIL-HTC), solvent degasser (DGU-20A3 prominence) and a temperature controlled compartment for column (CTO10AVP) were used for all the analytes. The chromatographic system consisted of Eclipse XDB-C18 (150 mm × 4.6 mm, 5m) analytical column bought from Agilent Company (USA). The flow rate of the mobile phase was kept at 0.5 mL/min. The autosampler temperature was set at 4°C and the injection volume was 10L. The mobile phase consisted of A (0.1% formic acid in

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