The rapid spread of NDM-1-positive bacteria proved to be a major challenge for the treatment and control of infectious diseases. The aim of our study was to confirm the presence of Carbapenem-hydrolyzing beta-lactamases in the Slovak Republic determinated with molecular-based detection methods. This paper is the first report of MBL NDM-1 in Slovakia. We describe four patients infected by multiresistant strain of Klebsiella pneumoniae and hospitalized on I.st Department of Anaesthesiology and Intensive Care Unit of the University Hospital L. Pasteur Kosice. In these cases the New Delhi metallo-beta-lactamase 1-producing Enterobacteriaceae was rapidly detected by phenotypic and genotypic methods. The rapid spread of NDM-1-positive bacteria proved to be a major challenge for the treatment and control of infectious diseases.
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