SummaryEpithelia grow and undergo extensive rearrangements to achieve their final size and shape. Imaging the dynamics of tissue growth and morphogenesis is now possible with advances in time-lapse microscopy, but a true understanding of their complexities is limited by automated image analysis tools to extract quantitative data. To overcome such limitations, we have designed a new open-source image analysis toolkit called EpiTools. It provides user-friendly graphical user interfaces for accurately segmenting and tracking the contours of cell membrane signals obtained from 4D confocal imaging. It is designed for a broad audience, especially biologists with no computer-science background. Quantitative data extraction is integrated into a larger bioimaging platform, Icy, to increase the visibility and usability of our tools. We demonstrate the usefulness of EpiTools by analyzing Drosophila wing imaginal disc growth, revealing previously overlooked properties of this dynamic tissue, such as the patterns of cellular rearrangements.

Moral rhetoric plays a fundamental role in how we perceive and interpret the information we receive, greatly influencing our decision-making process. Especially when it comes to controversial social and political issues, our opinions and attitudes are hardly ever based on evidence alone. The Moral Foundations Dictionary (MFD) was developed to operationalize moral values in the text. In this study, we present MoralStrength, a lexicon of approximately 1,000 lemmas, obtained as an extension of the Moral Foundations Dictionary, based on Word-Net synsets. Moreover, for each lemma it provides with a crowdsourced numeric assessment of Moral Valence, indicating the strength with which a lemma is expressing the specific value. We evaluated the predictive potentials of this moral lexicon, defining three utilization approaches of increased complexity, ranging from lemmas' statistical properties to a deep learning approach of word embeddings based on semantic similarity. Logistic regression models trained on the features extracted from MoralStrength, significantly outperformed the current state-of-the-art, reaching an F1-score of 87.6% over the previous 62.4% (p-value< 0.01), and an average F1-Score of 86.25% over six different datasets. Such findings pave the way for further research, allowing for an in-depth understanding of moral narratives in text for a wide range of social issues.

Deriving prior polarity lexica for sentiment analysis -where positive or negative scores are associated with words out of context -is a challenging task. Usually, a trade-off between precision and coverage is hard to find, and it depends on the methodology used to build the lexicon. Manually annotated lexica provide a high precision but lack in coverage, whereas automatic derivation from pre-existing knowledge guarantees high coverage at the cost of a lower precision. Since the automatic derivation of prior polarities is less time consuming than manual annotation, there has been a great bloom of these approaches, in particular based on the SentiWordNet resource. In this paper, we compare the most frequently used techniques based on SentiWordNet with newer ones and blend them in a learning framework (a so called 'ensemble method'). By taking advantage of manually built prior polarity lexica, our ensemble method is better able to predict the prior value of unseen words and to outperform all the other SentiWordNet approaches. Using this technique we have built SentiWords, a prior polarity lexicon of approximately 155,000 words, that has both a high precision and a high coverage. We finally show that in sentiment analysis tasks, using our lexicon allows us to outperform both the single metrics derived from SentiWordNet and popular manually annotated sentiment lexica.