No abstract

UAS captured increased genetic variation compared with manual terminal height. There were small significant differences in ground filtering methods to extract plant structure. Higher resolution did not improve imagery informativeness with regard to plant height. Logistic function provides informative phenotypes for temporal maize growth. Correlation and prediction accuracy of grain yield increased by ∼20% with UAS heights. Weekly unmanned aerial system (UAS) imagery was collected over the College Station, TX, 2017 Genomes to Fields (G2F) hybrid trial, across three environmental stress treatments, using two UAS platforms. The high‐altitude (120‐m) fixed‐wing platform increased the fraction of variation attributed to genetics and had highly repeatable (R > 60%) height estimates, increasing the genetic variance explained (10–40%) over traditional terminal plant height measurement (PHTTRML ∼30%), as well as over the low‐altitude rotary‐wing UAS platform (10–20%). A logistic function reduced the dimensionality (>20 flights) of each UAS dataset to three parameters (inflection point, growth rate, and asymptote) and produced a more robust predictive model than independent flight dates, effectively summarizing (R2 > 0.98) the UAS flight dates. The logistic model overcame the need to use specific flight dates when comparing different environments. The UAS height estimates (r = 0.36–0.48) doubled the correlations to grain yield in this G2F experiment compared with PHTTRML (r = 0.23–0.28). Parameters of the logistical function achieved equivalent correlations (r = 0.30–0.46) to individual flight dates (r = 0.36–0.48), improving grain yield prediction by ∼400% (R2 = 0.25–0.34) over PHTTRML (R2 = 0.06–0.08). Incorporating other UAS‐derived parameters beyond plant height may allow yield to be accurately predicted before maturity, speeding breeding programs. A new public R function to generate ESRI shapefiles for plot research is also described.

Core Ideas We comprehensively validated the use of UAS in sorghum and maize breeding programs. Temporal estimates of plant growth will allow researchers to elucidate new phenotypes. The stage of the breeding pipeline dictates the applicability of UAS platforms. The implementation of UAS is demonstrated in different crop species. Monetary and time costs should be considered before implementation of UAS. To meet future world food and fiber demands, plant breeders must increase the rate of genetic improvement of important agricultural crops. One of the biggest obstacles now facing crop scientists is a phenotyping bottleneck. To ease this burden, the emerging technology of unmanned aerial systems (UAS) presents an exciting opportunity. To assess the utility of UAS, it is important to investigate their application across multiple crop species. Terminal plant height is of great importance to maize (Zea mays L.) and sorghum [Sorghum bicolor (L.) Moench] breeders and has been hypothesized to be useful but has been logistically impractical to measure in the field. In this study, we statistically analyzed in depth the ability of UAS to estimate height in sorghum (advanced and early generation material) and maize (optimal and late material) and the application of these estimates in breeding programs. We found that UAS explain genotypic variation similarly to ground‐truth methods and that the repeatability of the methodology is high (R = 0.61–0.99), indicating effective differentiation of genotypes. Additionally, correlations between ground truth and UAS measurements were moderate to high for all materials (r = 0.4–0.9). Finally, we present a novel application for the technology in the form of high‐resolution temporal growth curves. Using these UAS‐generated growth curves, new physiological insights can be obtained and new avenues of scientific investigation are possible.

Spatially continuous estimates of forest aboveground biomass (AGB) are essential to supporting the sustainable management of forest ecosystems and providing invaluable information for quantifying and monitoring terrestrial carbon stocks. The launch of the Ice, Cloud, and land Elevation Satellite-2 (ICESat-2) on September 15th, 2018 offers an unparalleled opportunity to assess AGB at large scales using along-track samples that will be provided during its three-year mission. The main goal of this study was to investigate deep learning (DL) neural networks for mapping AGB with ICESat-2, using simulated photon-counting lidar (PCL)-estimated AGB for daytime, nighttime, and no noise scenarios, Landsat imagery, canopy cover, and land cover maps. The study was carried out in Sam Houston National Forest located in south-east Texas, using a simulated PCL-estimated AGB along two years of planned ICESat-2 profiles. The primary tasks were to investigate and determine neural network architecture, examine the hyper-parameter settings, and subsequently generate wall-to-wall AGB maps. A first set of models were developed using vegetation indices calculated from single-date Landsat imagery, canopy cover, and land cover, and a second set of models were generated using metrics from one year of Landsat imagery with canopy cover and land cover maps. To compare the effectiveness of final models, comparisons with Random Forests (RF) models were made. The deep neural network (DNN) models achieved R2 values of 0.42, 0.49, and 0.50 for the daytime, nighttime, and no noise scenarios respectively. With the extended dataset containing metrics calculated from Landsat images acquired on different dates, substantial improvements in model performance for all data scenarios were noted. The R2 values increased to 0.64, 0.66, and 0.67 for the daytime, nighttime, and no noise scenarios. Comparisons with Random forest (RF) prediction models highlighted similar results, with the same R2 and root mean square error (RMSE) range (15–16 Mg/ha) for daytime and nighttime scenarios. Findings suggest that there is potential for mapping AGB using a combinatory approach with ICESat-2 and Landsat-derived products with DL.

Continuing population growth will result in increasing global demand for food and fiber for the foreseeable future. During the growing season, variability in the height of crops provides important information on plant health, growth, and response to environmental effects. This paper indicates the feasibility of using structure from motion (SfM) on images collected from 120 m above ground level (AGL) with a fixed-wing unmanned aerial vehicle (UAV) to estimate sorghum plant height with reasonable accuracy on a relatively large farm field. Correlations between UAV-based estimates and ground truth were strong on all dates (R2 > 0.80) but are clearly better on some dates than others. Furthermore, a new method for improving UAV-based plant height estimates with multi-level ground control points (GCPs) was found to lower the root mean square error (RMSE) by about 20%. These results indicate that GCP-based height calibration has a potential for future application where accuracy is particularly important. Lastly, the image blur appeared to have a significant impact on the accuracy of plant height estimation. A strong correlation (R2 = 0.85) was observed between image quality and plant height RMSE and the influence of wind was a challenge in obtaining high-quality plant height data. A strong relationship (R2 = 0.99) existed between wind speed and image blurriness.

National Aeronautics and Space Administration’s (NASA’s) Ice, Cloud, and land Elevation Satellite-2 (ICESat-2) provides rich insights over the Earth’s surface through elevation data collected by its Advanced Topographic Laser Altimeter System (ATLAS) since its launch in September 2018. While this mission is primarily aimed at capturing ice measurements, ICESat-2 also provides data over vegetated areas, offering the capability to gain insights into ecosystem structure and the potential to contribute to the sustainable management of forests. This study involved an examination of the utility of ICESat-2 for estimating forest aboveground biomass (AGB). The objectives of this study were to: (1) investigate the use of canopy metrics for estimating AGB, using data extracted from an ICESat-2 transect over forests in south-east Texas; (2) compare the accuracy for estimating AGB using data from the strong beam and weak beam; and (3) upscale predicted AGB estimates using variables from Landsat multispectral imagery and land cover and canopy cover maps, to generate a 30 m spatial resolution AGB map. Methods previously developed with simulated ICESat-2 data over Sam Houston National Forest (SHNF) in southeast Texas were adapted using actual data from an adjacent ICESat-2 transect over similar vegetation conditions. Custom noise filtering and photon classification algorithms were applied to ICESat-2’s geolocated photon data (ATL03) for one beam pair, consisting of a strong and weak beam, and canopy height estimates were retrieved. Canopy height parameters were extracted from 100 m segments in the along-track direction for estimating AGB, using regression analysis. ICESat-2-derived AGB estimates were then extrapolated to develop a 30 m AGB map for the study area, using vegetation indices from Landsat 8 Operational Land Imager (OLI), National Land Cover Database (NLCD) landcover and canopy cover, with random forests (RF). The AGB estimation models used few canopy parameters and suggest the possibility for applying well-developed methods for modeling AGB with airborne light detection and ranging (lidar) data, using processed ICESat-2 data. The final regression model achieved a R2 and root mean square error (RMSE) value of 0.62 and 24.63 Mg/ha for estimating AGB and RF model evaluation with a separate test set yielded a R2 of 0.58 and RMSE of 23.89 Mg/ha. Findings provide an initial look at the ability of ICESat-2 to estimate AGB and serve as a basis for further upscaling efforts.

No abstract

Unoccupied aerial systems (UAS) were used to phenotype growth trajectories of inbred maize populations under field conditions. Three recombinant inbred line populations were surveyed on a weekly basis collecting RGB images across two irrigation regimens (irrigated and non-irrigated/rain fed). Plant height, estimated by the 95th percentile (P95) height from UAS generated 3D point clouds, exceeded 70% correlation (r) to manual ground truth measurements and 51% of experimental variance was explained by genetics. The Weibull sigmoidal function accurately modeled plant growth (R 2 : >99%; RMSE: <4 cm) from P95 genetic means. The mean asymptote was strongly correlated (r 2 = 0.66-0.77) with terminal plant height. Maximum absolute growth rates (mm/day) were weakly correlated with height and flowering time. The average inflection point ranged from 57 to 60 days after sowing (DAS) and was correlated with flowering time (r 2 = 0.45-0.68). Functional growth parameters (asymptote, inflection point, growth rate) alone identified 34 genetic loci, each explaining 3-15% of total genetic variation. Plant height was estimated at one-day intervals to 85 DAS, identifying 58 unique temporal quantitative trait loci (QTL) locations. Genomic hotspots on chromosomes 1 and 3 indicated chromosomal regions associated with functional growth trajectories influencing flowering time, growth rate, and terminal growth. Temporal QTL demonstrated unique dynamic expression patterns