Horseradish is a perennial plant with significant antioxidant properties, and it contains about 0.2% to 1.0% of essential oil, mainly sinigrin, sinigrin-derived allylisothiocyanate and diallylsulphide. The aim of the study was to determine composition of volatile compounds of horseradish (A. rusticana L.) roots depending on the genotype. Volatiles from fresh horseradish roots of nine genotypes were extracted using solid phase microextraction with DVB/Car/PDMS fibre and were further analysed using gas chromatography-mass spectrometry. The volatile compounds were identified by comparing their mass spectra with mass spectral libraries (Nist98) and by calculating linear retention indexes and comparing them with the literature data. The studied horseradish genotypes differed both in the quantitative and qualitative content of aroma compounds. Totally 15 volatile compounds were detected, and their highest amount was found in genotype G12B. The main aroma compound of all horseradish samples was allylisothiocyanate, which formed 64-82% of the total identified volatile compounds. The obtained results were compared with those found in the literature. All horseradish samples contained significant amounts of phenylethylisothiocyanate (4-18%) that is formed from glucosinolate - gluconasturtin. The study revealed that genotype has great influence on the content of volatiles in horseradish roots.

Horseradish contains many bioactive compounds with antioxidant activity. The current study aimed to evaluate the effect of various wall materials and their ratios on the physical properties and bioactive-compound retention and stability in microencapsulated horseradish leaf and root juices. Horseradish juice was microencapsulated using maltodextrin, maltodextrin/gum Arabic, soy protein isolate, and starch with three different core-to-wall ratios. The total phenolic, total flavonoid, total flavan-3-ol, and total phenolic-acid contents, as well as antioxidant activity, were determined using spectrophotometric methods, whereas individual phenol profiles were determined by high-performance liquid chromatography (HPLC). Multivariate analysis of variance showed that plant material, wall material, and core-to-wall ratio had a significant effect on the bioactive-compound retention and antioxidant-activity preservation. Microcapsules produced from horseradish leaf juice had a significantly higher content of phenolic compounds and antioxidant activity compared to root-juice microcapsules. However, better retention was observed for microencapsulated horseradish root juice. Maltodextrin and maltodextrin/gum Arabic were the most effective wall materials for the retention of bioactive compounds, while they also had a smaller particle size and better solubility. The horseradish-juice microcapsules possess a high content of rutin. The highest stability of bioactive compounds after storage was found at a core-to-wall ratio of 20:80.

Horseradish (Armoracia rusticana) leaves pomace, which contains high-value bioactive compounds, is the product resulting from pressing horseradish leaves for juice production. The aim of the current research was to investigate the effect of convective, microwave-vacuum and freeze-drying on the content of bioactive compounds in horseradish leaves pomace. Convective hot air-drying was performed at 40, 60 and 80 °C. The total phenolic content (TPC), total flavonoid content (TFC), total flavan-3-ol content, total phenolic acid content, total flavonol content, chlorophylls and total carotenoids, and antioxidant activity were determined by spectrophotometric methods. Individual profiles of phenols and organic acids are estimated by high-performance liquid chromatography (HPLC), but volatile compounds are estimated by gas chromatography (GC). Totally, 14 individual phenolic compounds, 8 organic acids, and 49 volatile compounds were analysed in the studied samples. The main phenolic compound identified in horseradish leaves pomace was rutin (3231 mg/100 g DW), among organic acids—quinic and malic acids, and volatile compounds—allyl isothiocyanate, 3-butenenitrile and benzyl alcohol. In the drying process, the content of some (total flavan-3-ols, total carotenoids content) compounds increased, but others (TPC, total organic acids content) decreased, and it was drying method-dependent. Freeze-drying caused the reduction of TPC by 29%, whereas convective drying by 53–59%. Fresh pomace contains such isothiocyanates as allyl isothiocyanate and butyl isothiocyanate, which were completely lost in the drying process. Freeze-drying allowed the best retention of various phenolic and volatile compounds in horseradish leaves pomace.

A perennial herb horseradish (Armoracia rusticana L.) contains biologically active substances and cultivated in temperate regions of the world. The aim of the current research was to determine the content of phenolic compounds and antioxidant properties of horseradish leaves depending on the harvest time. For experiments horseradish leaves three years at six different times (during the period from May to October) were collected. Fresh plant material was extracted with ethanol using conventional extraction. For all extracts total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity (DPPH˙, ABTS˙+ scavenging activity, reducing power) were determined using a spectrophotometric methods. Results showed that content of phenolic compounds and antioxidant activity of horseradish leaves were significantly affected by harvest time. The highest TPC and ABTS˙+ scavenging activity was observed in the horseradish leaves collected in May, but the highest TFC and antioxidant activity (DPPH radical scavenging activity and reducing power) was observed in June. The content of phenolics compounds and antioxidant activity significantly decreased during post flowering stage and continues till October. Horseradish leaves contained considerable amount of phenolics compounds and natural antioxidants. In further experiments, use of horseradish leaves as natural antioxidants in different food matrixes should be studied.

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Horseradish (Armoracia rusticana L.) is a perennial herb belonging to the Brassicaceae family; it contains biologically active substances such as phenolic compounds.The aim of the present research was to clasify horseradish root genotypes, based on the total phenol content and antioxidant properties, using the hierarchical cluster analysis (HCA), and to compare them with clusters obtained from data of the molecular random amplified polymorphic DNA (RAPD) analysis.Plant phenolic compounds are among the most important primary antioxidants. The phenolic composition of plants is affected by different factors such as variety, genotype, climate, harvest time, storage, processing. Nine genotypes of horseradish roots harvested at three different times in the period from August to November 2011 were used. Several statistical methods can be used to assess differences in the horseradish genotypes. Using a univariate statistical analysis and standard deviations for each analyzed variable does not help to get a complete insight into the complex analysis. Multivariate statistical methods are appropriate tools for the analysis of a complex data matrix. The hierarchical cluster analysis (HCA) used in the current research is a simple way of grouping the set of available data by their similarities according to a set of selected variables. No similarities were found by clustering the genotypes according to the content of biologically active compounds and molecular analyses.

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Plant pigments have a wide range of nutritional benefits. Chlorophyll has antioxidant, anti-inflammatory, heavy metal chelating etc. properties, whereas carotenoids exhibit significant antioxidant activities. The aim of current research was to determine the content of chlorophyll a, chlorophyll b and total carotenoids of frozen horseradish leaves and horseradish leave by-products depending on the used extraction solvent. For experiments, frozen horseradish leaves and horseradish leave by-products after juice extraction were extracted with four different solvents (acetone, diethyl ether, methanol and ethanol). Chlorophyll a, chlorophyll b and total carotenoids were determined spectrophotometrically at various wavelengths (470, 645 and 662 nm). Additionally, total chlorophyll content and ratio between chlorophyll a and b were calculated. Results showed that content of photosynthetic pigments in tested samples significantly (p<0.05) differed between used extraction solvents. The degree of extraction of these pigments is greatly influenced by their different chemical structures. For extraction of chlorophyll a and total carotenoids the best solvent was methanol in both cases (horseradish leaves and leave by-products). But acetone was the best solvent for extraction of chlorophyll b. Generally, chlorophyll a was detected in larger amounts in all analysed samples, better solvent was acetone, and the highest content of photosynthetic pigments were observed in horseradish leave by-products after juice extraction.