An ion chromatographic method was developed for the determination of nitrate and nitrite in vegetable and fruit baby foods. The introduction of nitrate or nitrite to food may be natural or artificial as a preservative. Because of the higher pH found in babies' stomachs, nitrate can act as a reservoir for the production of nitrite by nitrate-reducing bacteria that can be harbored in the intestinal tract. This problem does not exist in adults because of the lower pH of the adult stomach. Exposure to nitrite by infants can result in methemoglobinemia (blue baby syndrome). There are also indications that carcinogenic nitrosamines can be formed from nitrates at the higher pH. These gastric conditions disappear at approximately 6 months of age. In this method, nitrate and nitrite were separated on a hydroxide-selective anion exchange column using online electrolytically generated high-purity hydroxide eluant and detected using suppressed conductivity detection. Average recoveries of spiked nitrite residue ranged from 91 to 104% and spiked nitrate residue ranged from 87 to 104%. This method and the AOAC Official Method yield comparable results for samples containing incurred nitrate residue. In addition, this method eliminates the hazardous waste associated with the use of cadmium found in the AOAC Official Method.
An ion chromatographic method with post-column derivatization and spectrophotometric detection is presented for the determination of nitrate and nitrite (NOx) in baby food. NOx residues found naturally or added as preservatives were extracted from baby foods and determined by using ion chromatography with post-column derivatization and spectrophotometric detection. Nitrate was reduced to nitrite online by post-column reduction using vanadium(III) chloride and heat. Nitrite reacted with Griess reagent to produce a dye that was detected at 525 nm. The use of V(III) and heat to promote the reduction of nitrate to nitrite online is a novel feature of this detection system. The determination of incurred NOx residues in samples by using AOAC Method 993.03 yielded results comparable to those obtained by ion chromatography with spectrophotometric detection. The toxic and carcinogenic metal cadmium used in the AOAC Method to reduce the nitrate to nitrite was avoided. The proposed method provides simultaneous determination of nitrate and nitrite. Average recoveries of nitrate and nitrite residues ranged from 82 to 107 for fortification levels of 25400 ppm.
Accurate analysis of vitamins is essential to help the public maintain adequate intakes of vitamins. Currently in Atlanta Center for Nutrient Analysis (ACNA), AOAC Method 999.15 with fluorescence detection is utilized for the analysis of vitamin K in infant formulas, dietary supplements and other medical foods. An UHPLC-(+)-APCI-MS/MS method for vitamin K1 (phylloquinone) was developed to improve the accuracy, selectivity and eciency of the analysis. SRM1849a and infant formula samples were used to demonstrate that vitamin K1 data by LC-MS/MS analysis matched well with those from the AOAC Method 999.15. A single-laboratory validation of an UHPLC-MS/MS method for vitamin K1 analysis in SRM1849a showed good accuracy with a mean value of 99.6% of the certified value (n = 8). Recoveries of vitamin K1 at two dierent spike levels were 99.6 and 103.7% from SRM1849a. Mean recovery of vitamin K1 from four dierent infant formula samples was 102.4% ranging from 95.6 to 115.5% with %RSD of 7.8 15.6. Precision, measured as repeatability (%RSDr), was 8.7 for SRM1849a and ranged from 3.7 to 13.4 for four infant formula samples. Application of this method will help ACNA facilitate the accurate analysis of vitamin K in infant formulas and other samples.https://doi.org/10.21423/jrs-v03n02p027 (DOI assigned 8/7/2019)
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.