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A simple procedure for large scale isolation of Solanum tuberosum tuber lectin from potato starch industry waste water has been developed. The procedure employed magnetic chitosan microparticles as an affinity adsorbent. Magnetic separation was performed in a flow-through magnetic separation system. The adsorbed lectin was eluted with glycine/HCl buffer, pH 2.2. The specific activity of separated lectin increased approximately 27 times during the isolation process.
In this work we investigate the possibilities of the use of Horizontal Low Gradient Magnetic Field (HLGMF) (<100 T/m) for filtration, control and separation of the synthesized magnetic particles, considering, the characteristics of the suspension, the size and the type of nanoparticles (NPs) and focusing on the process scale up. Reversible aggregation is considered in the different steps of magnetic nanobeads synthesis. For these purpose, we synthesized Fe 2 O 3 -silica coreshell nanobeads by co-precipitation, monodispersion and silica coating. SQUID, TEM, XRD, and Zeta potential techniques were used to characterize the synthesized nanobeads. An extensive magnetophoresis study was performed at different magnetophoretic conditions. Different reversible aggregation times were observed at different HLGMF, at each step of the synthesis route: Several orders of magnitude differences where observed when comparing citric acid (CA) suspension with silicon coated beads. Reversible aggregation times are correlated with the properties of the NPs at different steps of synthesis.
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