No abstract

Pre-eclampsia (PE) is a pregnancy-specific disease characterized by the occurrence of hypertension and proteinuria after two weeks of gestation. Long noncoding RNAs (lncRNAs) are emerging as key regulators in PE development.This study aims to investigate the role of lncRNA, small nucleolar RNA host gene 5 (SNHG5), in the pathogenesis of PE. The expression of SNHG5 was significantly downregulated in placental tissues from patients with severe PE compared normal controls. Overexpression of SNHG5 promoted trophoblast (HTR-8/SVneo) cell proliferation, invasion, and migration, and flow cytometry results showed that SNHG5 overexpression inhibited apoptosis and caused a decrease of cell population at the G 0 /G 1 phase and an increase of cell population at the S phase, while knockdown of SNHG5 had the opposite effects. The interaction between SNHG5 and miR-26a-5p was predicted by bioinformatics analysis and confirmed by luciferase reporter assay and RNA immunoprecipitation, and miR-26a-5p was negatively regulated by SNHG5; miR-26a-5p expression was upregulated in PE placental tissues and was inversely correlated with SNHG5 expression. Furthermore, miR-26a-5p was predicted to target the 3′ untranslated region of N-cadherin, which was confirmed by luciferase reporter assay, and miR-26a-5p overexpression suppressed N-cadherin expression in HTR-8/SVneo cells. N-cadherin mRNA expression was downregulated in PE placental tissues and was positively correlated with SNHG5 expression.Both overexpression of miR-26a-5p and knockdown of N-cadherin suppressed HTR-8/SVneo cell invasion and migration, and also attenuated the effects of SNHG5 on the cellular functions of HTR-8/SVneo cells. In conclusion, our study suggested that SNHG5 promotes trophoblast cell proliferation, invasion, and migration at least partly via regulating the miR-26a-5p/N-cadherin axis. K E Y W O R D Sinvasion and migration, miR-26a-5p, N-cadherin, pre-eclampsia (PE), small nucleolar RNA host gene 5 (SNHG5), trophoblast J Cell Biochem. 2019;120:3173-3184.wileyonlinelibrary.com/journal/jcb

According to the report by the World Health Organization, depression, as the leading disabling disease in the world, has aroused widespread social concern. However, the shortage of medical and nursing staff in psychiatry conflicts with the rising nursing needs of depressed patients. First, depression is a chronic mental illness, its cure course is relatively long, and stable drug treatment and healthy living habits are keys to the curing process. Second, the disease feature where depression patients have stronger emotional needs and more sensitive mental states is ignored in existing health monitoring systems. In consideration of the above two aspects, a health management assistant is proposed in this paper, called MEMO box system, that focuses on emotion and with smart medicine box as carrier. Specifically, the MEMO box system is composed of electronic medicine box and smart applications on mobile device, and electronic medicine box can collect the multi-mode data of patients, including their medication behaviors, daily activities, physical exercise data, and so on, which provide data basis for the health assistant. Emotion recognition and exercise recommend algorithms are deployed in the edge cloud, which can quickly respond to health service requests from patients. With the cooperation of hardware and software in this system, patients are urged to take medicines on time to effectively control their conditions, and emotion cognition and exercise adjustment recommendation can be realized for depression patients, thus providing patients with empathic sports recommendations.

Background: Video endoscopic inguinal lymphadenectomy (VEIL) has fewer complications than open surgery for vulva cancer. No high-level evidence comparing the efficacy and safety of the two routes of VEIL (VEIL-H and VEIL-L) is available. Methods: We performed a comprehensive literature search for reports on VEIL for vulva cancer from 2000 to 2020 and reviewed our experience. Data extraction includes patients' characteristics, perioperative indicators, postoperative complications, and cancer recurrence.Results: A total of 11 eligible studies were included in the systematic review. Nine studies included only one route of VEIL, and the other two studies directly compared the two routes of VEIL. The average operation time of the VEIL-L group was 85 (range, 33-180.12) minutes, and that of the VEIL-H group was 112 (range, 35-170.79) minutes. The average blood loss of the VEIL-L group was 9 (range, 5-30) mL, and that of the VEIL-H group was 96 (range, 5.5-214.8) mL. The average number of intraoperatively removed lymph nodes in the VEIL-L group was 9 (range, 7.5-13.2), and that in the VEIL-H group was 14 (range, 9.5-16). No significant difference was observed in the cancer recurrence rate between the two groups. We found similar results in our cohort study. Conclusions:The operation time, intraoperative blood loss, and time of drainage of the VEIL-L procedure seemed less than those of the VEIL-H procedure. VEIL-L and VEIL-H were equivalent in the efficacy of lymph node dissection, surgery-related complications, and cancer recurrence rate.