Delay in detecting sepsis pathogens still become problematic issue for determining the definitive antibiotics therapy for the causative pathogens. The gold standard method for sepsis is blood culture but it requires 3 days to detect definitive pathogen. Molecular methods offer rapid identification of pathogens. We evaluated the use of sepsis flow chip (SFC) assay for identifying pathogens from children with sepsis. The blood samples from children with sepsis were collected and incubated in the culture machine. The positive samples were subjected to amplification-hybridization using sepsis flow chip assay and culture. A total of 94 samples from 47 patients were recovered, with 25 isolates were recovered, including K. pneumoniae (11), and S. epidermidis (6). From 25 positive blood culture bottles subjected to sepsis flow chip assay, there were 24 genus/species and 18 resistance genes detected. The sensitivity, specificity, and conformity was 80%, 94.2%, and 94.68% respectively. Sepsis flow chip assay offer promising assay to identify pathogens from positive blood culture in pediatric patient with sepsis and may support the antimicrobial stewardship program in hospital.
Delay in detecting sepsis pathogens still become problematic issue for determining the definitive antibiotics therapy for the causative pathogens. The gold standard method for sepsis is blood culture but it requires 3 days to detect definitive pathogen. Molecular methods offer rapid identification of pathogens. We evaluated the use of sepsis flow chip (SFC) assay for identifying pathogens from children with sepsis. The blood samples from children with sepsis were collected and incubated in the culture machine. The positive samples were subjected to amplification-hybridization using sepsis flow chip assay and culture. A total of 94 samples from 47 patients were recovered, with 25 isolates were recovered, including K. pneumoniae (11), and S. epidermidis (6). From 25 positive blood culture bottles subjected to sepsis flow chip assay, there were 24 genus/species and 18 resistance genes detected. The sensitivity, specificity, and conformity was 80%, 94.2%, and 94.68% respectively. Sepsis flow chip assay offer promising assay to identify pathogens from positive blood culture in pediatric patient with sepsis and may support the antimicrobial stewardship program in hospital.
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