INTRODUCTIONAs a typical water-soluble vitamin, vitamin B2 (riboflavin) is an essential micronutrient for human beings. It plays an important role in the human body [1][2][3][4] . Riboflavin deficiency may lead to skin and mucosal disorders, angular cheilitis, anemia or glossitis and seborrheic dermatitis 5 . Therefore, a simple, sensitive and accurate determination of riboflavin is very important. Different methods have been used for the determination of riboflavin, such as high performance liquid chromatography (HPLC)-photodiode array detection 6 , HPLC-fluorescence spectroscopy 7-9 , HPLC-matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (HPLC-MALDI-TOF) 10 and capillary electrophoresis (CE) laser induced fluorescence (LIF) spectroscopy 11,12 . To our best of knowledge, no methods to date have been published for the determination of riboflavin in food by HPLC with in-column optical fiber laser-induced fluorescence detection technology. In this work, the HPLC separation of vitamin B2 in foods and the ICOF-LIF detection system are studied and optimized. This method is simple, high sensitivity and good precision. EXPERIMENTALTangerine and mushroom were purchased from local markets (Chengdu, China). HPLC-grade methanol and VB2
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