Light and gibberellins (GAs) mediate many essential and partially overlapping plant developmental processes. DELLA proteins are GA-signalling repressors that block GA-induced development 1 . GA induces degradation of DELLA proteins via the ubiquitin/ proteasome pathway 2 , but light promotes accumulation of DELLA proteins by reducing GA levels 3 . It was proposed that DELLA proteins restrain plant growth largely through their effect on gene expression 4,5 . However, the precise mechanism of their function in coordinating GA signalling and gene expression remains unknown. Here we characterize a nuclear protein interaction cascade mediating transduction of GA signals to the activity regulation of a light-responsive transcription factor. In the absence of GA, nuclear-localized DELLA proteins accumulate to higher levels, interact with phytochrome-interacting factor 3 (PIF3, a bHLH-type transcription factor) and prevent PIF3 from binding to its target gene promoters and regulating gene expression, and therefore abrogate PIF3-mediated light control of hypocotyl elongation. In the presence of GA, GID1 proteins (GA receptors) elevate their direct interaction with DELLA proteins in the nucleus, trigger DELLA protein's ubiquitination and proteasome-mediated degradation, and thus release PIF3 from the negative effect of DELLA proteins.Light and GA interact during Arabidopsis thaliana seedling development, regulating hypocotyl elongation, cotyledon opening and light-responsive gene expression; their pathways seem to converge at regulation of the abundance of DELLA proteins (GA pathway repressors) 3,6 . Arabidopsis has five DELLA proteins-RGA, GAI, RGL1, RGL2 and RGL3-defined by their unique DELLA domain and a conserved GRAS domain 4 . To analyse them in vivo, we raised antibodies against endogenous RGA and generated transgenic Arabidopsis expressing each of the five DELLA proteins with tandem affinity purification (TAP) tags ( Supplementary Fig. 1). The response of DELLA protein levels to exogenously applied GA 3 (an active form of GA) or PAC (paclobutrazol, a GA biosynthesis inhibitor) was examined. We found that one-hour-long GA treatment eliminates the majority of DELLA proteins, and this GA effect can be largely prevented by 100 mM MG132 (a 26S proteasome-specific inhibitor). PAC, on the other hand, promotes over-accumulation of DELLA proteins (Fig. 1). These results show for the first time in Arabidopsis that all the DELLA proteins are under negative control by GA and the proteasome. Next, we generated lines expressing TAPtagged RGAD17 and GAID17, which lack a 17 amino acid motif within the DELLA domain that is required for GA-induced degradation 7,8 . As expected, TAP-RGAD17 and TAP-GAID17 are completely resistant to GA and accumulate at higher levels than wild-type proteins, which cannot be further increased by PAC (Fig. 1, and *These authors contributed equally to this work. WTAnti-RPN6Anti-RGA Immunoblot analysis of RGA (by anti-RGA antibody) and TAP-DELLA proteins (by anti-MYC antibody) in various light-grown Ara...
In animals, the sphingolipid metabolite sphingosine-1-phosphate (S1P) functions as both an intracellular messenger and an extracellular ligand for G-protein-coupled receptors of the S1P receptor family, regulating diverse biological processes ranging from cell proliferation to apoptosis. Recently, it was discovered in plants that S1P is a signalling molecule involved in abscisic acid (ABA) regulation of guard cell turgor. Here we report that the enzyme responsible for S1P production, sphingosine kinase (SphK), is activated by ABA in Arabidopsis thaliana, and is involved in both ABA inhibition of stomatal opening and promotion of stomatal closure. Consistent with this observation, inhibition of SphK attenuates ABA regulation of guard cell inward K(+) channels and slow anion channels, which are involved in the regulation of stomatal pore size. Surprisingly, S1P regulates stomatal apertures and guard cell ion channel activities in wild-type plants, but not in knockout lines of the sole prototypical heterotrimeric G-protein alpha-subunit gene, GPA1 (refs 5, 6, 7-8). Our results implicate heterotrimeric G proteins as downstream elements in the S1P signalling pathway that mediates ABA regulation of stomatal function, and suggest that the interplay between S1P and heterotrimeric G proteins represents an evolutionarily conserved signalling mechanism.
To elucidate genome-level responses to drought and high-salinity stress in rice, a 70mer oligomer microarray covering 36,926 unique genes or gene models was used to profile genome expression changes in rice shoot, flag leaf and panicle under drought or high-salinity conditions. While patterns of gene expression in response to drought or high-salinity stress within a particular organ type showed significant overlap, comparison of expression profiles among different organs showed largely organ-specific patterns of regulation. Moreover, both stresses appear to alter the expression patterns of a significant number of genes involved in transcription and cell signaling in a largely organ-specific manner. The promoter regions of genes induced by both stresses or induced by one stress in more than one organ types possess relative enrichment of two cis-elements (ABRE core and DRE core) known to be associated with water stress. An initial computational analysis indicated that novel promoter motifs are present in the promoters of genes involved in rehydration after drought. This analysis suggested that rice might possess a mechanism that actively detects rehydration and facilitates rapid recovery. Overall, our data supports a notion that organ-specific gene regulation in response to the two abiotic stresses may primarily be mediated by organ-specific transcription responses.
The phytohormone gibberellic acid (GA) regulates diverse aspects of plant growth and development. GA responses are triggered by the degradation of DELLA proteins, which function as repressors in GA signaling pathways. Recent studies in Arabidopsis thaliana and rice (Oryza sativa) have implied that the degradation of DELLA proteins occurred via the ubiquitinproteasome system. Here, we developed an Arabidopsis cell-free system to recapitulate DELLA protein degradation in vitro. Using this cell-free system, we documented that Lys-29 of ubiquitin is the major site for ubiquitin chain formation to mediate DELLA protein degradation. We also confirmed the specific roles of GA receptors and multisubunit E3 ligase components in regulating DELLA protein degradation. In addition, blocking DELLA degradation with a PP1/PP2A phosphatase inhibitor in our cell-free assay suggested that degradation of DELLA proteins required protein Ser/Thr dephosphorylation activity. Furthermore, our data revealed that the LZ domain of Arabidopsis DELLA proteins is essential for both their stability and activity. Thus, our in vitro degradation system provides biochemical insights into the regulation of DELLA protein degradation. This in vitro assay system could be widely adapted for dissecting cellular signaling pathways in which regulated proteolysis is a key recurrent theme.
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