Herein we report a reversed‐phase high‐performance liquid chromatography tandem mass spectrometry (RP‐HPLC/MS/MS) method for the analysis of positional isomers of triacylglycerols (TAGs) in vegetable oils. The fragmentation behavior of [M + X]+ ions (X = NH4, Li, Na or Ag) was studied on a quadrupole‐time‐of‐flight (Q‐TOF) mass spectrometer under low‐energy collision‐induced dissociation (CID) conditions. Mass spectra that were dependent on the X+ ion and the nature and position of the acyl substituents were observed for four pairs of 'AAB/ABA'‐type TAGs, namely PPO/POP, OOP/OPO, LLO/LOL and OOL/OLO (where P is 16:0, palmitic acid; O is 18:1, oleic acid; and L is 18:2, linoleic acid). For the majority of [M + X]+ adducts, the loss of the fatty acid in the outer positions (sn‐1 or sn‐3) was favored over the loss in the central position (sn‐2), which enabled the determination of the fractional abundance of the isomers. Ratios of the intensity of fragment ions at various AAB/ABA compositions produced linear calibration curves with positive slopes, comparable to those obtained traditionally by ESI‐MS/MS of [M + NH4]+ adducts. The only exceptions were the [M + Ag]+ adducts of the PPO/POP system, which produced calibration curves with negative slopes. Sodium adducts provided the most consistent level of isomeric discrimination for the TAGs studied and also offered the most convenience in that they required no additive to the mobile phase. Therefore, calibration curve data derived from [M + Na]+ adducts were applied to the quantification of TAG regioisomers in sunflower and olive oils. The regiospecific analysis showed that palmitic acid was typically located at positions sn‐1 or sn‐3, whereas unsaturated fatty acids, oleic and linoleic acids were mostly found at the sn‐2 position. Copyright © 2010 Crown in the right of Canada. Published by John Wiley & Sons, Ltd.
The behavior in atmospheric pressure chemical ionization of selected model polycyclic aromatic compounds, pyrene, dibenzothiophene, carbazole, and fluorenone, was studied in the solvents acetonitrile, methanol, and toluene. Relative ionization efficiency and sensitivity were highest in toluene and lowest in methanol, a mixture of molecular ions and protonated molecules was observed in most instances, and interferences between analytes were detected at higher concentrations. Such interferences were assumed to be caused by a competition among analyte molecules for a limited number of reagent ions in the plasma. The presence of both molecular ions and protonated analyte molecules can be attributed to charge-transfer from solvent radical cations and proton transfer from protonated solvent molecules, respectively. The order of ionization efficiency could be explained by incorporating the effect of solvation in the ionization reactions. Thermodynamic data, both experimental and calculated theoretically, are presented to support the proposed ionization mechanisms. The analytical implications of the results are that using acetonitrile (compared with methanol) as solvent will provide better sensitivity with fewer interferences (at low concentrations), except for analytes having high gas-phase basicities. (J Am Soc Mass Spectrom 2008, 19, 1926 -1941) © 2008 Published by Elsevier Inc. on behalf of American Society for Mass Spectrometry T he analysis of polycyclic aromatic compounds (PACs) in petroleum and environmental samples has been the subject of many investigations [1-3], due to their impact on the environment, refining processes, and product quality. However, success is often hindered by the number and low concentration of these compounds in such samples, as well as by the lack of standards. Gas chromatography (GC) and GC coupled with electron ionization (EI) mass spectrometry (GC/ MS) are normally employed for samples containing robust, thermally stable analytes [4,5]. Atmospheric pressure ionization methods [6]-electrospray ionization (ESI) [7], atmospheric pressure chemical ionization (APCI) [8], and atmospheric pressure photoionization (APPI) [9]-in conjunction with liquid chromatography (LC) have been used for samples of more problematic analytes.It is generally believed that ESI suffers more from nonlinear response and matrix effects than APCI. Linear response over a wide concentration range is an attractive feature of APCI and has been reported by several authors [10,11]. Recently, Roussis and Fedora compared the ability of APCI and ESI to quantify polar and ionic compounds in petroleum products [12]. They obtained linear ranges of three orders of magnitude for both techniques, and higher sensitivity for ESI. However, the ESI response was nonlinear over the concentration range of interest, and they recommended the use of APCI for quantitative LC/MS applications.In the course of a study of the application of APCI to the analysis of nitrogen-and particularly sulfurcontaining PACs in petroleum samples, we observe...
Software, available at no cost on the Internet, is described which uses polynomial expansion algorithms to calculate the isotope patterns for precursor ion, neutral loss, and MSn product ion tandem mass spectra. Such information is useful for determining product ion and neutral loss composition, identification of analytes in complex samples, deconvolution of overlapping spectra, and correction of peak heights or areas in quantitative analysis. The effect of less than unit mass resolution on the isotope patterns is described and experimental examples of the use of the software are presented.
Analysis of triacylglycerols (TAGs), found as complex mixtures in living organisms, is typically accomplished using liquid chromatography, often coupled to mass spectrometry. TAGs, weak bases not protonated using electrospray ionization, are usually ionized by adduct formation with a cation, including those present in the solvent (e.g., Na(+)). There are relatively few reports on the binding of TAGs with cations or on the mechanisms by which cationized TAGs fragment. This work examines binding efficiencies, determined by mass spectrometry and computations, for the complexation of TAGs to a range of cations (Na(+), Li(+), K(+), Ag(+), NH4(+)). While most cations bind to oxygen, Ag(+) binding to unsaturation in the acid side chains is significant. The importance of dimer formation, [2TAG + M](+) was demonstrated using several different types of mass spectrometers. From breakdown curves, it became apparent that two or three acid side chains must be attached to glycerol for strong cationization. Possible mechanisms for fragmentation of lithiated TAGs were modeled by computations on tripropionylglycerol. Viable pathways were found for losses of neutral acids and lithium salts of acids from different positions on the glycerol moiety. Novel lactone structures were proposed for the loss of a neutral acid from one position of the glycerol moiety. These were studied further using triple-stage mass spectrometry (MS(3)). These lactones can account for all the major product ions in the MS(3) spectra in both this work and the literature, which should allow for new insights into the challenging analytical methods needed for naturally occurring TAGs.
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