What limits phytoplankton growth in nature? The answer is elusive because of methodological problems associated with bottle incubations and nutrient addition experiments. We are investigating the possibility that antibodies to proteins repressed by a specific nutrient can be used as probes to indicate which nutrient limits photosynthetic carbon fixation in the ocean. The diatom Phaeodactylum tricornutum Bohlin and the chlorophyte Dunaliella tertiolecta Butcher were grown in batch cultures in artificial seawater and f/2 nutrient lacking either phosphorus, iron, or nitrogen. Chlorosis was induced by nutrient limitation in both species with the exception of phosphorus‐limited D. tertiolecta. The synthesis and appearance of specific proteins were followed by labeling with 14C‐bicarbonate. Nutrient limitation in general leads to a decrease in the quantum efficiency of photosystem II, suggesting that deficiency of any nutrient affects the photosynthetic apparatus to some degree: however, the effect of nitrogen and iron limitation on quantum efficiency is more severe than that of phosphorus. A crude fractionation of the soluble and membrane proteins demonstrated that the large proteins induced under limitation by phosphorus and iron were associated with the membranes. However, small iron‐repressible proteins were located in the soluble fraction. Isolation with anion‐exchange chromatography and N‐terminal sequencing of iron‐repressible, 23‐kDa Proteins from D. tertiolecta, P. tricornutum, and Chaetoceros gracilis revealed that these small soluble proteins have strong homology with the N‐terminal sequence of flavodoxins from Azotobacter and Clostridium. The identity of the flavodoxin from D. tertiolecta was confirmed by immunodetection using antiflavodoxin raised against Chlorella. Flavodoxin was detected only under iron deprivation and was absent from nitrogen‐and phosphorus‐limited algae. Flavodoxin is a prime candidate for a molecular probe of iron limitation in the ocean. The requirements to confirm its utility in nature are discussed.

Using a national probability sample of municipal police departments, this study provides the first systematic look at the prevalence, predictors, and content of municipal police websites in the United States. A content analysis revealed that police agencies with websites (42% of all police agencies nationwide in 2008) were more inclined to use websites to “push out” information rather than “pull in” information from the community. The results support the hypothesis that departments with a greater emphasis on community policing (defined by Law Enforcement Management and Administrative Statistics [LEMAS] data) would be more likely to have a website, exhibit greater transparency in the display of data and provide more opportunities for citizen input. Crime rate and population size were also important determinants of website creation and content, respectively. This study concludes that websites are underutilized by both the police and research communities.

No abstract

The purpose of this study was: (1) to assess the impact of an incident of racial profiling on residents' attitudes about profiling; and (2) to examine the effects of exposure to a video clip of deliberation about the incident on residents' beliefs about the causes of profiling. All residents, White and minority, were less likely to believe that Chicago police officers engaged in profiling after the incident. These findings suggest that attitudes about the prevalence of racial profiling are susceptible to the manner in which the media construct incidents of police misconduct. Exposure to the video clip was not related to differences in residents' beliefs about the causes of profiling, but was related to differences in perceptions of the dangerousness of traffic stops. The findings highlight the need for more research on how media constructions of police misconduct influence attitudes about profiling and impact community-police relations.Research findings highlight the salience of race in predicting attitudes toward police, particularly that minorities tend to hold more negative views of police than

A protein unique to phosphorus stress observed in Dunaliella tertiolecta Butcher was studied in the context of phosphate‐limited cell physiology and is a potential diagnostic indicator of phosphate deficiency in this alga. Cells were grown over a range of limited, steady‐state growth rates and at maximum (replete) and zero (phosphate‐starved) growth rates. The stress protein, absent in nutrient‐replete cells, was produced under all steady‐state phosphate‐limited conditions and increased in abundance with increasing limitation (decreasing growth rate). Cellular carbon: phosphorus ratios and the maximum uptake rate of phosphate (Vm) increased with increasing limitation, whereas the ratio of chlorophyll a: carbon decreased. Alkaline phosphatase activity did not respond to limitation but was measurable in starved, stationary‐phase cells. Fv/Fm, a measure of photochemical efficiency, was a nonlinear, saturating function of p, as commonly observed under N limitation. The maximum Fv/Fm of 0.64 was measured in nutrient‐replete cells growing at μmax, and a value of zero was measured in stationary‐phase starved cells. When physiological parameters were compared, the P‐stress protein abundance and Fv/Fm were the most sensitive indicators of the level of deficiency. The stress protein was not produced under N‐ or Fe‐limited conditions. It is of high molecular weight (>200) and is associated with internal cell membranes. The stress protein has several characteristics that make it a potential diagnostic indicator: it is 1) unique to phosphorus limitation (i.e. absent under all other conditions), 2) present under limiting as well as starved conditions, 3) sensitive to the level of limitation, and 4) observable without time‐course incubation of live samples.

Purpose The purpose of this paper is to provide a systematic review of the literature examining the role of news media consumption and awareness in shaping public attitudes about police. Design/methodology/approach A comprehensive, systematic search of multiple academic databases (e.g. EBSCO Host) was undertaken, supplemented by the use of Google Scholar to search among journals indicated as having cited the articles found in the databases. Findings A total of 42 studies were identified that met the selection criteria for this meta-review and examined exposure to high-profile incidents involving police, awareness of negative news coverage of police, and/or consumption of specific news mediums (e.g. newspapers). Overall, research supports a relationship between negative perceptions of police and both exposure to high-profile incidents and awareness of negative coverage. Some support for the influence of consuming television news on attitudes exists, but more research is needed on the role of different news sources in shaping perceptions. Future research should also include determining causal pathways and how news about police is selected. Originality/value This is the first meta-review of the research examining how news media and attitudes about police are related. This study will provide a useful resource for those researchers wishing to continue to examine different aspects of news media consumption as a predictor of perceptions.

No abstract

Purpose Given the heightened scrutiny of police by the media in the post-Ferguson era, the purpose of this paper is to test hypotheses derived from the cultivation theory regarding possible media-related effects on perceptions of police legitimacy. Design/methodology/approach A sample of 1,197 residents from a mid-size California city was surveyed. Regression analyses were conducted to examine the relative effects of media consumption and personal experience on perceptions of police legitimacy. Findings Partial support for the cultivation theory was found. Those who reported local TV as their most important news source saw police as more legitimate than those who reported the internet as most important. Consistent with past research, procedural justice was the strongest predictor of perceptions of police legitimacy for those recently stopped by the police. Awareness of negative media depictions of police, however, also had independent effects indicating that media consumption does impact perceptions of police legitimacy. Originality/value While a wealth of research on the relationship between procedural justice and perceptions of police legitimacy exists, no previous research has examined the role media consumption plays in shaping such perceptions.