This study aims to determine the effect of using Cassia alata L. leaf extract on the prevention of fungus on fish eggs Pangasianodon hypophthalmus This research was conducted at the Blangpidie Fish Seed Center. Aceh Barat Daya from July to August 2020. Statistical analysis used a completely randomized design (CRD) method with 7 treatment levels and 3 replications. The treatments were conducted by soaking the fish eggs in the leaf extract for 20 minutes. Those treatments were A (control), B (2.5 ppm), C (5 ppm), D (7.5 ppm) E (10 ppm), F (12.5 ppm), and G (15 ppm). ANOVA test results showed that the addition of leaf extract C. alata had a significant (P <0.05) effect on hatchability and survival of P. hypophthalmus larvae. A concentration of 15 ppm is the best with a hatchability value of 85.33% and a concentration of 10 ppm is the most optimum for the survival value of P. hypophthalmus larvae, namely 92.99%.
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