The rapid progression of DNA technology allows for the application of recently developed techniques to an ever-growing body of archaeological and environmental material recovered from submerged archaeological sites. As NGS and DNA Capture replace PCR as the predominant method used to characterise DNA present within an archaeological sample, it is necessary to consider how this effects the possibilities for future research, but also gives cause to reconsider the findings of previously published work from PCR experiments on archaeological material from submerged sites. In this review, the pitfalls, promise, and future directions of ancient DNA research on archaeological material from coastal and maritime sites are discussed. Here, it is argued that a common stumbling block in past research has been a lack of characterisation of the deposition environment, and that this has obscured our understanding of DNA identified at submerged sites. Unique aspects of the marine environment may present further complications in our attempts to authenticate ancient DNA. Overcoming these challenges will significantly enhance our ability to confidently assign an archaeological origin to DNA isolated in artefacts, organic remains, and sediments from submerged archaeological sites.
Despite growing evidence to the contrary, wine remains the assumed content of many types of ancient pottery. Vessels from the Kyrenia and Mazotos shipwrecks, and Yeronisos island presumed to have contained wine were subjected to three different extraction protocols to test the assumption that these vessels were used to import and serve wine. Chemical extracts reveal grapevine products but also other fruit juice, beeswax and plant oil, sometimes mixed with grapevine products due to intentional mixing or reuse. Biomarkers detected in sediment samples from Mazotos and Yeronisos demonstrate why quantification is vital. Analyses show that even seemingly identical ceramics from the same shipwreck contained different commodities.
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