Dysarthria is universal in Parkinson's disease (PD) during disease progression; however, the quality of vocalization changes is often ignored. Furthermore, the role of changes in the acoustic parameters of phonation in PD patients remains unclear. We recruited 35 PD patients and 26 healthy controls to perform single, double, and multiple syllable tests. A logistic regression was performed to differentiate between protective and risk factors among the acoustic parameters. The results indicated that the mean f0, max f0, min f0, jitter, duration of speech and median intensity of speaking for the PD patients were significantly different from those of the healthy controls. These results reveal some promising indicators of dysarthric symptoms consisting of acoustic parameters, and they strengthen our understanding about the significance of changes in phonation by PD patients, which may accelerate the discovery of novel PD biomarkers. Abbreviations PD Parkinson's disease HKD Hypokinetic dysarthria VHI-30 Voice Handicap Index H&Y Hoehn-Yahr scale UPDRS III Unified Parkinson's Disease Rating Scale Motor Score Parkinson's disease (PD), a chronic, progressive neurodegenerative disorder with an unknown etiology, is associated with a significant burden with regards to cost and use of societal resources 1,2. More than 90% of patients with PD suffer from hypokinetic dysarthria 3. Early in 1969, Darley et al. defined dysarthria as a collective term for related speech disorders. The classification of dysarthria includes flaccid dysarthria, spastic dysarthria, ataxic dysarthria, hypokinetic dysarthria, hyperkinetic dysarthria, unilateral upper motor neuron dysarthria and mixed dysarthria 4. The speech abnormalities of patients with PD are collectively termed hypokinetic dysarthria (HKD). These speech flaws are typically characterized by increased acoustic noise, a reduced intensity of voice, harsh and breathy voice quality, increased voice nasality, monopitch, monoloudness, speech rate disturbances, the imprecise articulation of consonants, the involuntary introduction of pauses, the rapid repetitions of words and syllables and sudden deceleration or acceleration in speech. Speech impairments are caused by impaired speech mechanisms during any of the basic motor processes involved in speech performance 5. The neuromotor speech sequence activates the muscles of the pharynx, tongue, larynx, chest and diaphragm through subthalamic secondary pathways. The anatomical substrate that could result in the abnormalities of PD phonetics may be reduced by the poor coordination of the sound-making muscles 6 .