In intracytoplasmic sperm injection (ICSI), a single sperm cell is selected and injected into an egg. The quality of the chosen sperm and specifically its DNA fragmentation have a significant effect on the fertilization success rate. However, there is no method today to measure the DNA fragmentation of live and unstained cells during ICSI. We present a new method to predict the DNA fragmentation of sperm cells using multi-layer stain-free imaging data, including quantitative phase imaging, and lightweight deep learning architectures. The DNA fragmentation ground truth is achieved by staining the cells with acridine orange and imaging them via fluorescence microscopy. Our prediction model is based on the MobileNet convolutional neural network architecture combined with confidence measurement determined by distances between vectors in the latent space. Our results show that the mean absolute error for cells with high prediction confidence is 0.05 and the 90th percentile mean absolute error is 0.1, where the range of DNA fragmentation score is [0,1]. In the future, this model may be applied to improve cell selection by embryologists during ICSI.
Smartphone mode classification is essential to many applications, such as daily life monitoring, healthcare, and indoor positioning. In the latter, it was shown that knowledge of the smartphone location on pedestrians can improve the positioning accuracy. Most of the research conducted in this field is focused on pedestrian motion in a horizontal plane. In this research, we use supervised machine learning techniques to recognize and classify the smartphone mode (text, talk, pocket and swing) while accounting for the movement up and downstairs. We distinguish between the going up and the down motion, each with four different smartphone modes, making eight states in total. This classification is based on the use of an optimal set of sensors that varies according to battery life and the energy consumption of each sensor. The classifier was trained and tested on a dataset constructed from multiple user measurements (total of 94 min) to achieve robustness. This provided an accuracy of more than 90% in the cross validation method and 91.5% if the texting mode is excluded. When considering only stairs motion, regardless of the direction, the accuracy improves to 97%. These results may assist many algorithms, mainly in pedestrian dead reckoning, in improving a variety of challenges such as speed and step length estimation and cumulative error reduction.
Intracytoplasmic sperm injection (ICSI) requires precise selection of a single sperm cell in a dish to be injected into an oocyte. This task is challenging due to high sperm velocity, collision with other sperm cells, and changes in the imaging focus. Herein, a new model is proposed, which is based on a multilayer long short‐term memory (LSTM) network combined with linear extrapolation calculation, for predicting the future location of individual sperm cells based on their previous paths. The model is trained with a unique loss function, constructed from the predicted location and trajectory, and results in low mean location error predictions. The results are based on comparing different input sequences length, number of time frames ahead, and motility parameters of sperm cells. This model can provide faster and more accurate sperm motility predictions, better tracking, and aid automatic sperm capturing technologies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.