Siberian wild rye (Elymus sibiricus L.), an allotetraploid species, is a potentially high-quality perennial forage crop native to temperate regions. We used fluorescently conjugated oligonucleotides, representing ten repetitive sequences, including 6 microsatellite repeats, two satellite repeats, and two ribosomal DNAs, to characterize E. sibiricus chromosomes, using sequential fluorescence in situ hybridization and genomic in situ hybridization assays. Our results showed that microsatellite repeats (AAG) 10 or (AGG) 10 , satellite repeats pAs1 and pSc119.2, and ribosomal 5S rDNA and 45S rDNA are specific markers for unique chromosomes. A referable karyotype ideogram was suggested, by further polymorphism screening, across different E. sibiricus cultivars with a probe mixture of (AAG) 10 , Oligo-pAs1, and Oligo-pSc119.2. Chromosomal polymorphisms vary between different genomes and between different individual chromosomes. In particular, two distinct forms of chromosome E in H genome were identified in intra-and inter-populations. Here, the significance of these results, for E. sibiricus genome research and breeding, and novel approaches to improve fluorescence in situ hybridization-based karyotyping are discussed.

Phosphorus (P) is one of the essential nutrients for alfalfa (Medicago sativa L.) growth, but P deficiency in soil is a common phenomenon. Applying an appropriate amount of P fertilizer for alfalfa in the establishment year improves winter survival, helps achieve sustainable high yields, and promotes the best economic returns. The objective of this study was to determine the effect of different P fertilization rates (0, 22, 44, and 66 kg P ha−1) on winter survival, forage yield, and root traits of a dormant and semi-dormant alfalfa cultivar in the establishment and first production year. Our results showed that applying an appropriate amount of P fertilizer can increase forage yield and enhance the persistence of late-summer seeded alfalfa by promoting the development of crown and root traits and improving winter survival, especially for semi-dormant cultivars. Late-summer seeding and application of appropriate P fertilizer rates are effective agronomic methods for alfalfa in cold and arid winter regions, and can be used as strategies to improve fertility and stabilize soil from wind erosion for sustainable agriculture. To obtain high rate of survival in winter, the optimal P fertilization rates for the dormant and semi-dormant alfalfa cultivars in this study were 31.4 and 41.3 kg P ha−1, whereas the optimum P application rates for high forage yields were 31.3 and 31.5 kg P ha−1, respectively.