Several tillage and planting methods have been proposed to enhance the soil bulk density, biological community, and grain yield of rice. In this work, we present the impact of plowing methods with different rice crop establishment approaches, i.e., moldboard plowing with mechanical transplanting (MPMT), rotary tillage with mechanical transplanting (RTMT), moldboard plowing with direct seeding (MPDS), and rotary tillage with direct seeding (RTDS), on soil bulk density, microbial community, enzymatic activities, and grain yield of ratoon rice (RR). The results showed that MPDS improved soil bulk density in 0–30 cm depth in both years and both harvesting times (1H: 1st harvest and 2H: 2nd harvest). The results also showed that microbial community significantly improved under MPDS compared to the other treatments in both years and in 1H and 2H. Additionally, enzymatic activities showed a positive effect under MPDS in both years and in 1H and 2H. MPDS subsequently improved rice grain yield by 18.05% and 17.27% in 2017 (1H and 2H), and 14.86% and 18.64% in 2018 (1H and 2H), respectively. In conclusion, MPDS appears to be a more suitable approach to obtaining high soil eminence and health, as well as sustainable RR production.

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