Embryonic miscarriage severely affects the life quality and physical and mental state of pregnant women. However, the detailed mechanism underlying embryonic miscarriage is not fully understood. This study is aimed at analyzing embryonic miscarriage. We collected samples from 25 normal pregnant women and 25 embryonic miscarriage patients of similar age to analyze microbiota isolated from the vagina. Crude examination of the vagina isolates showed that compared with the control group, 80% of the embryonic miscarriage group contained a significantly lower number of Lactobacillus, the major healthy microbe in the vagina. Furthermore, the levels of Th1 and Th2 secreted cytokine interleukin 2 (IL-2) and interleukin10 (IL-10), respectively, were examined. Results showed that the IL2 level was higher, and IL10 level was lower in the embryonic miscarriage group than in the control group, whereas the IL2/IL10 level was higher in the embryonic miscarriage group than in the control group. This finding suggested that the immune response was suppressed in the embryonic miscarriage group. To further dissect the microbiota of the vagina in the two groups, 16S rDNA sequencing was performed. Bioinformatics analysis showed that 1096 and 998 overlapped operational taxonomic units were identified from the embryonic miscarriage and control groups, respectively. At the genus level, the relative abundance of Fam_Finegoldia, Lac_Coprococcus_3, and Lac_Roseburia significantly differed in the embryonic miscarriage group. Overall, our analyses provided potential biomarkers for embryonic miscarriage and elucidated the causative relationship between microbiota and immune responses and may enable the possible diagnosis and therapeutics of early pregnancy loss.
A novel water-stable metal-organic framework based on In(III), namely, [In 3 (btta) 2 -(OH) 3 (H 2 O)]⋅(DMA) 2 (1, DMA = N,, constructed from a novel ligand (2E,2 ′ E,2 ″ E)-3,3 ′ ,3 ″ -(benzene-1,3,5-triyl)triacrylic acid (H 3 btta), was obtained by a solvothermal reaction and structurally characterized by X-ray diffraction of single crystal along with elemental analysis. Owing to its high porosity and insensitive luminescent emission at room temperature, it was studied as a light-emitting sensor for detecting toxic Cr 2 O 7 2− anion in water, which shows high selectivity and sensitivity. To solve the bacterial problem, the protective effect of the compound against bacterial infection after missed abortion was evaluated. In the in vivo model, after the treatment with the compound, the clonal formation unit (CFU) in the uterine tissue was evaluated, and the animal weight was measured and recorded. In addition to this, the level of IL-18, C-reactive protein, TNF-, as well as IL-1 within the infectious tissues was detected by the ELISA method. Pose scoring software and molecular docking showed that the compound might bind to the NF-B protein, and this might offer a latent regulatory mechanism for the compound. K E Y W O R D S bacterial infection, Cr 2 O 7 2sensing, In(III)-complex, inflammatory response, missed abortion, molecular docking, porous framework, thermal shift 178
A new luminescent In(III)-based coordination polymer with the chemical formula of {[In(ipa)(H2O)](H2O)(CH3CN)2}n (1, H3ipa = 5-hydroxyisophthalate) has been successfully prepared via a solvothermal reaction of H3ipa and In(NO3)3·6H2O in CH3CN and DMF mixed solvent. The strong emission of 1 could be quenched efficiently by trace amounts of Cr2O72−, while other competing analogues show no such quenching effect, indicating complex 1 could be applied as an effective sensor for the detection of Cr2O72− in the solution. To solve the problem of bacterial infection after missed abortion, the protective effect of the compound in vivo was further explored. CFU detection was carried to evaluate the inhibitory effect of compound on bacterial numbers, and then ELISA was conducted to detect the level of IL-18 and IL-1β in the infectious tissues. The pharmacokinetic detection of the compound was also performed in SD rats. The results obtained from molecular docking and pose scoring software exhibited a possible binding mode of the compound binding to NFκB, which might provide potential regulation mechanisms for the compound.
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